雌激素对脑卒中后抑郁大鼠海马和杏仁核脑源性生长因子-磷酸化酪氨酸激酶B表达以及行为学的影响

被引：16

作者：

蒋慧刚 ^{[1
,2
]}

肖丽 ^{[1
,2
]}

张红霞 ^{[1
,2
]}

李九如 ^{[1
,2
]}

王骏阳 ^{[1
,2
]}

金坤林 ^{[1
,3
,2
]}

程建华 ^{[1
]}

邵蓓 ^{[1
,2
]}

机构：

[1] 温州医学院附属第一医院神经内科

[2] 温州医学院附属第一医院浙江省神经老化与疾病研究重点实验室

[3] 温州医学院附属第一医院神经外科

来源：

中国临床神经科学 | 2014年 / 22卷 / 02期

关键词：

雌激素; 抑郁; 脑卒中; 酪氨酸激酶B; 脑源性生长因子; 海马; 杏仁核;

D O I：

暂无

中图分类号：

R749.1 [脑器质性精神障碍];

学科分类号：

100204 [神经病学];

摘要：

目的通过观察雌激素对缺血性脑卒中后抑郁大鼠模型海马和杏仁核的脑源性生长因子(BDNF)-磷酸化酪氨酸激酶B(pTrkB)的表达,探讨缺血性脑卒中后内源性抑郁的发病机制。方法将SD雌性大鼠随机分为对照组12只(无任何干预)、模型组16只(MCAO术后2周,皮下注射大豆油,持续2周)和雌激素组16只(MCAO术后2周,皮下注射溶有10μg 17β-雌二醇的0.1 mL大豆油,持续2周)。通过旷场实验和强迫游泳实验观察大鼠行为学变化,应用免疫组化和Western blot方法观察海马和杏仁核中BDNF和pTrkB表达。结果雌激素干预后:①旷场实验和强迫游泳中,大鼠行为学评分均显著提高,雌激素组与模型组比较,差异有统计学意义(P<0.01);②免疫组化法观察示雌激素组海马和杏仁核BDNF阳性细胞数与模型组比较明显增多,差异有统计学意义(P<0.05);③Western blot法检测示雌激素组BDNF/β-actin和pTrkB/TrkB灰度比值较模型组明显提高(P<0.05)。结论雌激素能改善脑卒中后大鼠的抑郁行为,其机制可能是通过BDNF-pTrkB信号通路的变化而改善PSD症状。

引用

页码：146 / 153

页数：8

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