大鼠短暂性局灶性脑缺血早期HMGB1在半暗带区的表达

被引：4

作者：

王鄂友 ^{[1
]}

朱正华 ^{[2
]}

朱萧玲 ^{[2
]}

夏中元 ^{[1
]}

熊利泽 ^{[2
]}

机构：

[1] 武汉大学人民医院麻醉科

[2] 第四军医大学西京医院麻醉科

来源：

神经解剖学杂志 | 2011年 / 27卷 / 04期

关键词：

高迁移率族蛋白B1; 脑缺血; 细胞因子; 炎症; 半暗带; 大鼠;

D O I：

暂无

中图分类号：

R743.3 [急性脑血管疾病（中风）];

学科分类号：

100204 [神经病学];

摘要：

目的:探讨调节炎症反应的细胞因子HMGB1在脑缺血/再灌注损伤中的时程变化特点。方法:采用大鼠MCAO模型,利用ELISA及免疫组织化学染色方法观察缺血/再灌注后不同时间点脑内HMGB1蛋白的表达变化。结果:ELISA检测提示再灌注后大鼠缺血侧大脑HMGB1表达显著增高,在10 h和70 h形成两次高峰(P<0.05);免疫组织化学染色提示再灌注1 h后部分大鼠(1/5)和再灌注后4,10,22和70 h后全部大鼠梗死区周边半暗带区的细胞外间隙有HMGB1阳性染色颗粒,后期并在部分细胞内出现HMGB1高表达。结论:结果提示,HMGB1不仅与脑缺血/再灌注的晚期损伤有关,亦可能参与了早期损伤过程。

引用

收藏

页码：438 / 442

页数：5

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