抗真菌γ-硫堇蛋白Rs-afp1基因导入苹果获得转基因植株

被引：10

作者：

刘庆忠

赵红军

孙清荣

J.Ingersoll

F.A.Hammerschlag

机构：

[1] 山东省果树研究所

[2] 美国农业部Beltsville农业研究中心

[3] 美国农业部Beltsville农业研究中心泰安

[4] 泰安

来源：

农业生物技术学报 | 2001年 / 03期

关键词：

γ-硫堇蛋白; Rubisco激活酶; 抗真菌; 转基因苹果;

D O I：

暂无

中图分类号：

S661.103.53 []; Q943.2 [植物基因工程];

学科分类号：

090201 ; 071007 ; 090102 ;

摘要：

以“皇家嘎啦”（Ｒｏｙａｌ　Ｇａｌａ）苹果（Ｍａｌｕｓ　ｄｏｍｅｓｔｉｃａ　Ｂｏｒｋｈ）为试材，在绿色组织特异表达的菠菜核酮糖二磷酸羧化酶／加氧酶的激活酶（Ｒｕｂｉｓｃｏ－ａｃｔｉｖａｓｅ）启动子（ＲＣＡＰ）驱动下，将抗真菌γ－硫堇蛋白（γ－ｔｈｉｏｎｉｎ）Ｒｓ－ａｆｐ１基因和ｕｉｄＡ（ｇｕｓ）基因导入白化茎段外植体，获得了ｎｐｔⅡ抗性植株。转化体经ＰＣＲ和Ｓｏｕｔｈｅｒｎ　ｂｌｏｔｔｉｎｇ杂交检测，证实了γ－硫堇蛋白Ｒｓ－ａｆｐ１基因和ｇｕｓ基因已经整合到苹果的染色体组上。ｇｕｓ染色证实了ＲＣＡＰ启动子在苹果组织中的特异性表达。转基因植株已移栽于大田，进行农业性状鉴定。

引用

页码：239 / 242+307 +307

页数：5

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