淫羊藿总黄酮及淫羊藿素体外抗骨肉瘤的研究

被引：10

作者：

刘玉亮 ^{[1
,2
]}

梁广胜 ^{[1
,2
]}

魏强强 ^{[1
,2
]}

何丁文 ^{[1
,2
]}

殷明 ^{[1
]}

殷嫦嫦 ^{[3
]}

机构：

[1] 南昌大学第二附属医院骨一科

[2] 南昌大学研究生院医学部

[3] 九江学院医学部分析与检测实验室

来源：

中成药 | 2013年 / 35卷 / 10期

关键词：

淫羊藿; Saos2骨肉瘤细胞; 增殖抑制; 凋亡; 缺氧诱导因子;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的观察淫羊藿黄酮和淫羊藿素对人骨肉瘤细胞株Saos2以及缺氧诱导因子(HIF1α)、Caspase-3和Bcl-2基因表达水平影响。方法 CCK-8法检测各种浓度对Soas2细胞增殖的影响;倒置相差显微镜观察作用后肿瘤细胞的形态学变化;AnnexinV/PI双标记法流式细胞术检测淫羊藿素诱导Saos2细胞早期凋亡的情况;RT-PCR半定量检测HIF1α、Caspase-3、Bcl-2的表达量。结果淫羊藿总黄酮和淫羊藿素都可抑制Soas2细胞增殖,并可致早期凋亡,RT-PCR半定量检测到HIF1α、Bcl-2的表达量降低而Caspase-3增高。结论淫羊藿素能抑制骨肉瘤Soas2细胞的增殖与HIF1α表达下调相关,诱导其产生早期凋亡,与Caspase-3的高表达及Bcl-2表达下调有关。

引用

收藏

页码：2072 / 2077

页数：6

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