应用单克隆抗体及ABC法检测淋巴细胞亚群

被引:5
作者
杨荫昌
耿同超
机构
[1] 中国医学科学院北京协和医院神经科
关键词
单克隆抗体; 细胞层; 淋巴细胞亚群; 玻片标本; 细胞单层;
D O I
10.15932/j.0253-9713.1989.02.015
中图分类号
学科分类号
摘要
应用抗Leu系列鼠抗人总T细胞、辅助性T细胞、抑制性T细胞及B细胞表面抗原的单克隆抗体,以免疫过氧化物酶技术之一——ABC法检测56例正常国人外周血中以上淋巴细胞亚群的分布。本文详细介绍了实验方法,指出这一方法兼具单克隆抗体特异性强和ABC法敏感性高的特点,但也存在非特异性染色等影响,并讨论了消除这些影响的措施。
引用
收藏
页码:94 / 97
页数:4
相关论文
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