2010-2011无年形我体国的东感北染林情区况蜱调中查嗜吞噬细胞

被引：7

作者：

李颖 ^{[1
,2
]}

左双燕 ^{[2
,3
]}

唐琨 ^{[2
,3
]}

浦冠勤 ^{[1
]}

机构：

[1] 苏州大学金螳螂建筑与城市环境学院

[2] 军事医学科学院微生物流行病研究所

[3] 中南大学公共卫生学院

来源：

中国媒介生物学及控制杂志 | 2012年 / 23卷 / 02期

关键词：

嗜吞噬细胞无形体; 蜱; 16SrRNA基因; gltA基因;

D O I：

暂无

中图分类号：

R384.4 [螨、蜱];

学科分类号：

100103 ;

摘要：

目的调查我国东北林区蜱标本中嗜吞噬细胞无形体的感染情况。方法2010-2011年在东北林区采集蜱标本,用聚合酶链反应方法扩增gltA基因片段,分析东北林区蜱中无形体的感染情况,代表阳性片段联用长片段16S rRNA进行扩增测序确认;并将所得序列与GenBank中已注册的序列进行比对分析。结果共检测蜱2293只,其中2010年1161只,阳性率4.91%,2011年1132只,阳性率4.24%,2年的感染率差异无统计学意义(χ2=0.373,P=0.830;χ2=1.789,P=0.409)。全沟硬蜱阳性率4.86%(91/1872),长角血蜱为3.41%(14/411),森林革蜱为0(0/10),3个蜱种的感染率差异无统计学意义(P>0.05)。测得的gltA序列中,NE-gltA-1与Hc346株(GenBank:GU935788)最相似,仅差2 bp,相似性99%;NE-gltA-2与MDJ-Ip92株(HQ396224)序列一致,相似性100%。与2个序列相似性最远的是ZJ-HGA-72株(DQ458811),相差53 bp,相似性84%。测得的16S rRNA序列与我国从东北鼠源分离株China-C-T(tGQ412339)最接近,相似性为99%,与Florida株(AF309867)相似性最远,相似性97%。结论我国东北林区蜱中普遍存在嗜吞噬细胞无形体的感染,全沟硬蜱和长角血蜱可能为无形体的媒介宿主。

引用

页码：111 / 113+117 +117

页数：4

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