银染增强的纳米金探针技术检测微量核酸

被引:12
作者
赵立凡
李柏生
黑笑涵
李晓波
李媛媛
徐顺清
机构
[1] 华中科技大学同济医学院公共卫生学院环境医学研究所环境与健康教育部重点实验室
关键词
纳米金; 核酸检测; 银染增强;
D O I
10.13865/j.cnki.cjbmb.2006.11.012
中图分类号
Q52-3 [];
学科分类号
摘要
利用银染增强的纳米金技术建立了一种简单快速的核酸定量方法.该方法基于纳米金与烷巯基修饰的寡核苷酸分子共价键合作用,将纳米微粒报告基团标记在与靶核酸一端序列互补的寡核苷酸上,同时生物素化修饰另一端互补序列.靶核酸与两段寡核苷酸探针杂交后,借亲和素固定在酶标板孔内,通过纳米金催化的银染放大效应产生高灵敏的识别信号,适时记录其吸光度值从而实现核酸分子的定量.该检测方法检测单链核酸分子的灵敏度达0.1fmol/L,双链分子为10fmol/L.
引用
收藏
页码:919 / 923
页数:5
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