细胞因子对神经干细胞增殖和分化的影响

被引：9

作者：

张文治

苏心

秦进喜

孔繁明

孔建国

王新平

只达石

机构：

[1] 天津市环湖医院病理科

[2] 天津市环湖医院病理科天津

[3] 天津

来源：

临床与实验病理学杂志 | 2003年 / 01期

关键词：

人胎大脑神经干细胞; 上皮细胞生长因子; 碱性纤维母细胞生长因子; 分裂; 分化;

D O I：

10.13315/j.cnki.cjcep.2003.01.024

中图分类号：

R329.2 [人体细胞学];

学科分类号：

100101 ;

摘要：

目的　建立人胎大脑神经干细胞 (NSCs)分离培养的体外培养系统 ,鉴定其干细胞原始特征 ,观察上皮细胞生长因子(EGF)、碱性纤维母细胞生长因子 (FGF2 )和脑源神经生长因子 (BDNF)对NSCs分裂和分化的影响。方法　在含EGF和 (或 )FGF2的培养体系中分离培养NSCs。用定量RT PCR法检测干细胞巢蛋白表达丰度 ,用PCR ELISA法检测端粒酶活性 ,PC NA用免疫细胞化学染色检测干细胞增殖特征以及不同代数分裂的干细胞的比率。免疫细胞化学法证实NSCs分化后神经元和星形胶质细胞的比例趋势 (NF 2 0 0、MAP 2、synaptophysin、NeuN和GFAP染色 )。结果　NSCs大量增殖呈球体状 ,PCNA阳性率高达 (71 6± 4 9) %、干细胞巢蛋白阳性、端粒酶活性为 6 3%。不同诱导因子对NSCs球体内细胞增殖数差异有显著性(P <0 0 5 )。证实EGF和 (或 )FGF2在培养基质存在下均能诱导NSCs分化 ,脑源神经生长因子 (BDNF)能维持神经元的存活和生长。分化后的神经元比例可达 (18 6± 2 5 ) %。结论　EGF和 (或 )FGF2可诱导NSCs分裂增殖并保持原始特性 ,在培养基质存在条件下 ,这些细胞因子可诱导NSCs分化。

引用

页码：77 / 81

页数：5

共 13 条

[1]

Developmental Biology[C]. 2008年国际口腔及颅颌前沿研究研讨会,1600

[2]

Fibroblast growth factor-2 (FGF-2)promotes acquisition of epidermal growth fctor (EGF) responsiveness in mouse striatal precursor cells: Identification of neural precursors responding to both EGF and FGF-2. Ciccolini F,Svendsen CN. The Journal of Neuroscience . 1998

[3]

Isolation and cloning of multipotential stem cells from the embryonic human CNS and establishment of transplantable human neural stem cell lines by epigenetic stimulation. Vescovi AL,Parati EA,Gritti A,et al. Experimental Neurology . 1999

[4]

Insulin-like growth factor-I is necessary for neural stem cell proliferation and demonstrates distinct actions of epidermal growth factor and insulin-like growth factor-I. Arsenijeric Y,Weiss S,Schneider B,et al. The Journal of Neuroscience . 2001

[5]

[6]

A strategy for gene therapy and brain repair. Pinucs DW,Goodman RR,Fraser RA,et al. Neurosurgery . 1998

[7]

Multipotent and restricted precursors in the central nervous system. Rao MS. The Anatomical Record . 1999

[8]

Mitogen and substrate differentially affect the lineage restriction of adult rat subventricular zone neural precursor populations. Whittemore SR,Morassutti DJ,Walters WM,et al. Experimental Cell Research . 1999

[9]

Adult bone marrow stromal cells ditterentiation into neural cells in vitro. Sanchez R,Song S,Cardozo P,et al. Experimental Neurology . 2000

[10]

Collagen type Ⅳ promotes the differentiation of neuronal progenitors and inhibits astroglial differentiation in cortical cell culture. Ali SA,Pappas IS,Parnavelas JG. Developmental Brain Research . 1998

← 1 2 →