内皮祖细胞培养条件的正交设计研究

被引：6

作者：

张怀勤

季亢挺

杨德业

黄晓燕

林捷

黄伟剑

徐力辛

机构：

[1] 温州医学院第一附属医院心内科

[2] 温州医学院第一附属医院心内科浙江温州

[3] 浙江温州

来源：

温州医学院学报 | 2005年 / 04期

基金：

浙江省自然科学基金;

关键词：

内皮祖细胞; 细胞培养; 正交设计;

D O I：

10.13771/j.cnki.33-1386/r.2005.04.001

中图分类号：

R329 [人体组织学];

学科分类号：

100101 ;

摘要：

目的:研究内皮祖细胞(EPCs)培养的适宜条件。方法:以L827正交表安排影响FPCs扩增贴壁的几个主要因素,光镜下观察集落形成,流式细胞术分析CD34、VE-Cadherin双阳性细胞,以此为指标分析各因素对EPCs培养的影响。结果:血管内皮生长因子(VEGF)及接种密度对集落形成影响显著, 内皮细胞生长添加剂(ECGS)及纤维连接蛋白皿底包被无影响。4个因素对EPCs得率均有影响,且VEGF 及接种密度之间尚有交互作用。结论:EPCs培养的适宜条件为:皿底包被纤维连接蛋白,5×106/cm2接种密度,VEGF 10 ng/ml及ECGS 150μg/m1。

引用

页码：261 / 264

页数：4

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