黄芪三萜皂苷通过抑制TGF-β1/Smad2、p38MAPK信号抑制CVB3诱导的心肌纤维化

被引：12

作者：

张兰 ^{[1
]}

刘晶 ^{[1
]}

刘天龙 ^{[1
]}

张勇 ^{[2
]}

刘小玲 ^{[2
]}

肖云峰 ^{[3
]}

刘小雷 ^{[3
]}

机构：

[1] 内蒙古医科大学附属医院药剂科

[2] 内蒙古自治区人民医院药剂处

[3] 内蒙古医科大学药理学教研室

来源：

中国分子心脏病学杂志 | 2018年 / 18卷 / 05期

关键词：

黄芪三萜皂苷; CVB3; 心肌纤维化; TGF-β1/Smad2、p38MAPK信号;

D O I：

10.16563/j.cnki.1671-6272.2018.10.011

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的本文旨在研究黄芪三萜皂苷(astragalus saponins,AST)对CVB3诱导心肌纤维化的保护作用及机制。方法使用CVB3病毒和BALB/c小鼠制备病毒性心肌炎模型并使用AST进行干预。记录不同处理组小鼠的生存曲线、检测不同干预组小鼠外周血中CK-MB水平、心肌组织HE染色和天狼星红染色检测心肌损伤和纤维化水平及通过western blot和qPCR检测心肌组织中TGF-β1、Smad2及p38MAPK水平以研究AST的作用机制。结果 AST能够显著提高小鼠的生存率,显著降低CVB3诱导的心肌损伤和外周血中CK-MB水平。与CVB3组小鼠比较,AST干预组小鼠心肌组织纤维化面积显著减少。无论是RNA水平还是蛋白水平,CVB3能够显著诱导TGF-β1、Smad2及p38MAPK在心肌组织中的表达,而AST对此具有显著地抑制作用。结论 AST对CVB3诱导的心肌组织纤维化具有显著地抑制作用,这种保护作用与抑制CVB3诱导的TGF-β1/Smad2、p38MAPK信号在心肌组织中的活化有关。

引用

页码：2631 / 2634

页数：4

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