云杉未成熟合子胚诱导体细胞胚胎发生

被引：29

作者：

张建伟 ^{[1
]}

王军辉 ^{[1
]}

李青粉 ^{[1
]}

马建伟 ^{[2
]}

机构：

[1] 中国林业科学研究院林业研究所国家林业局林木培育重点实验室

[2] 甘肃省小陇山林业科学研究所

来源：

林业科学 | 2014年 / 50卷 / 04期

关键词：

云杉; 体细胞胚胎发生; 胚性愈伤组织; 成熟体细胞胚;

D O I：

暂无

中图分类号：

S791.18 [云杉];

学科分类号：

090702 [森林培育];

摘要：

以云杉未成熟合子胚为外植体,研究球果采集日期、基因型、植物生长素和细胞分裂素对愈伤组织诱导的影响,同时对影响体细胞胚分化和成熟的关键因素进行探讨。结果表明:合适的胚性愈伤组织诱导和增殖培养基为1/2 LM+2,4-D 2.2 mg·L-1+6-BA 1.1 mg·L-1+蔗糖10 g·L-1+凝胶2 g·L-1+酶解酪蛋白1 g·L-1;合适的分化培养基为1/2 LM+ABA 24 mg·L-1+PEG4000 50 g·L-1+蔗糖30 g·L-1+活性炭1 g·L-1+凝胶4 g·L-1+水解酪蛋白1 g·L-1;合适的萌发培养基为1/4 LM+蔗糖20 g·L-1+活性炭1 g·L-1+凝胶4 g·L-1+水解酪蛋白1 g·L-1。以上培养基中均附加谷氨酰胺0.5 g·L-1,pH5.8。

引用

页码：39 / 46

页数：8

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