木霉菌平板抗菌、几丁质酶和β-1,3-葡聚糖酶活性与病害防治效果

被引：19

作者：

杨合同

肖性龙

徐砚珂

机构：

[1] 山东省科学院中日友好生物技术研究中心

[2] 山东省科学院中日友好生物技术研究中心山东济南

[3] 山东济南

来源：

山东科学 | 2003年 / 02期

关键词：

木霉菌; 生物防治; 筛选; 几丁质酶; β-1,3-葡聚糖酶;

D O I：

暂无

中图分类号：

S476 [生物防治];

学科分类号：

0904 ;

摘要：

对60株分离自不同植物根际的木霉菌菌株(包括哈茨木霉菌,绿色木霉菌,康宁木霉菌和绿木霉菌)进行了平皿对峙试验、测定了几丁质酶和β-1,3-葡聚糖酶活性。所有菌株在这三个指标上均表现出明显的差异。选择38个抑菌能力、几丁质酶和β-1,3-葡聚糖酶活性不同的菌株进行的盆载试验表明,所有菌株对棉花立枯病都有防治效果,但不同菌株的防治效果差异显著。相关性分析表明,木霉菌防治棉花立枯病的效果与平皿对峙抑制率,几丁质酶和β-1,3-葡聚糖酶活性间没有数量上的相关关系。

引用

页码：1 / 6

页数：6

共 9 条

[1]

Endochitinase from Gliocladium virens :isolation, characterization, and synergistic antifungal activity in combination with gliotoxin. Pietro A D,Lorito M,Hayes C K,et al. Phytopathology . 1993

[2]

Microbial interactions and biocontrol in the rhizosphere. Whipps J M. J. Exp. Botany, 2001, 52(roots special issue) .

[3]

Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Zeilinger S,Galhaup C,Payer K,et al. Fungal Genetics and Biology . 1999

[4]

Colorimetric determination of reducing sugars in soils. Deng S P,Tabatabai M A. Soil Biology and Biochemistry . 1994

[5]

Autolysis and chitinase production in cultures of Verticillium albo-atrum. Vessey J C,Pegg G F. Transactions of the British Mycological Society . 1973

[6]

Analysis of the β-1,3-glucanolytic system of the biocontrol agent Trichoderma harzianum. Vazquez-Garciduenas S,Leal--Morales A A,Herrera-Estrella. Applied and Environmental Microbiology . 1998

[7]

Cell wall synthesis is a major target of mycoparasitic antagonism by Trichoderma harzianum. Lorito M,Farkas V,Rebuffat S,et al. Journal of Bacteriology . 1996

[8]

Synergistic combination of cell wall degrading enzymes and different antifungal compounds enhances inhibition of spore germination. Lorito M,Hayes C K,Di Pietro A,et al. Microbiology . 1994

[9]

Chitinolytic enzymes of Tricboderma harzianum:purification of chitobiosidase and endochitinase. Harman G E,Hayes C K,Lorito M,et al. Phytopathology . 1993

← 1 →