用于评价小球隐孢子虫卵囊感染力的NMRI乳鼠模型(英文)

被引:2
作者
Xunde LI
Philippe Brasseur
机构
[1] Laboratoire de Parasitologie
[2] Hospital Charles Nicolle !CHU
[3] 不详
[4] Rouen
[5] Franc
[6] 不详
关键词
隐孢子虫; 卵囊; 感染力;
D O I
暂无
中图分类号
R-332 [医用实验动物学];
学科分类号
摘要
[目的 ]评价一定数量的小球隐孢子虫卵囊的感染力。 [方法 ]4日龄 NMRI乳鼠经口腔管饲法接种不同数量的卵囊 ,7d后处死乳鼠 ,取消化道幽门至肛门段并匀成悬液 ,取此悬液置载玻片上 ,石碳酸品红染色 ,相差显微镜查见卵囊的为被感染乳鼠。以各组被感染乳鼠百分率评价卵囊的感染力。 [结果 ]接种 15 0 0或 2 0 0 0的乳鼠均被感染 ;接种 10 0 0、 5 0 0、2 5 0、 10 0和 5 0个卵囊的乳鼠 ,感染率分别为 88%、 74%、 5 1%、 2 8%和 9.5 %。 [结论 ]NMRI乳鼠模型接种卵囊数在 15 0 0~5 0范围内 ,其接种量与感染率呈正相关。该模型可用于评价一定数量小球隐孢子虫卵囊的感染力。
引用
收藏
页码:32 / 34
页数:3
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