TNF-α Up-regulates Matrix Metalloproteinase-9 Expression and Activity in Alveolar Macrophages from Patients with Chronic Obstructive Pulmonary Disease

被引：2

作者：

李亚清 ^{[1
]}

张珍祥 ^{[2
]}

徐永健 ^{[2
]}

熊盛道 ^{[2
]}

倪望 ^{[2
]}

陈仕新 ^{[2
]}

机构：

[1] Department of Respiratory Medicine, Zhejiang Provincial People's Hospital

[2] Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

来源：

华中科技大学学报(医学英德文版) | 2006年 / 06期

关键词：

chronic obstructive pulmonary disease; alveolar macrophage; matrix metalloproteinase; NF-kappa B;

D O I：

暂无

中图分类号：

R563.9 [其他];

学科分类号：

1002 ; 100201 ;

摘要：

To study the effects of tumor necrosis factor (TNF)-α on matrix metalloproteinase (MMP)-9 expression and activity in alveolar macrophages (AM) and to investigate the role of NF-κB in the induction, AM were collected from bronchoalveolar lavage fluid (BALF) of healthy subjects and patients with chronic obstructive pulmonary disease (COPD). MMP-9 expression and activity were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), West-ern blotting and zymography. NF-κB activity was detected by electrophoretic mobility shift assay (EMSA). MMP-9 expression and activity induced by TNF-α in AM from healthy subjects or patients with COPD were significantly increased in a dose-dependent manner (P<0.05). NF-κB activity in-duced by TNF-α was significantly increased in AM from patients with COPD, and pyrrolidine di-thiocarbamate (PDTC) and N-acetyl-L-cysteine (NAC) significantly inhibited the activation of NF-κB induced by TNF-α (P<0.05). The presents study suggested that the expression and activity of MMP-9 from AM can be induced by TNF-α, and TNF-α/NF-κB signal pathway may play an impor-tant role in the induction.

引用

页码：647 / 650

页数：4

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