HPLC/MS测定人血浆中盐酸班布特罗及其代谢物特布他林的浓度

被引：6

作者：

刘蕾

李可欣

史爱欣

殷琦

赫广威

孙春华

不详

机构：

[1] 卫生部北京医院临床药理基地!北京

来源：

药物分析杂志 | 2001年 / 05期

关键词：

班布特罗; 特布他林; 高效液相-质谱联用;

D O I：

10.16155/j.0254-1793.2001.05.004

中图分类号：

R969 [临床药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的 :用高效液相 /质谱联用法测定人血浆中盐酸班布特罗及其代谢物特布他林的浓度。方法 :液相 :采用SupelcodiscoveryC18色谱柱 (5 μm ,2 5 0mm× 4 6mm) ;柱温 40℃ ;流动相为甲醇 -醋酸铵溶液 (0 0 0 7mol·L-1) (2 0∶80 ) (并用冰醋酸调 pH =4 8) ,流速 0 6mL·min-1,进样量 6 0 μL ;质谱 :大气压化学电离源 (APCI) ,选择性监测 (SIR)质荷比 (m/z)分别为 2 2 6 (特布他林 ) ,2 6 0 (内标 ) ,36 8(班布特罗 )带正电荷的分子离子峰定量。样品用固相萃取小柱提取处理。结果 :班布特罗线性范围 0 12 5～ 16 μg·L-1,最低检测浓度为 0 0 5 μg·L-1。特布他林线性范围 0 312 5～ 40 μg·L-1,最低检测浓度为 0 0 5 μg·L-1。班布特罗和特布他林的萃取回收率均在 90 %以上 ,日内、日间的RSD皆小于 15 %。结论 :适用于临床上测定血浆中盐酸班布特罗及其代谢物特布他林的浓度及药动学的研究

引用

页码：316 / 319

页数：4

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