Paenibacillus polymyxa CP7菌抗革兰氏阴性菌活性组分的分离和结构分析

被引：9

作者：

陈海英 ^{[1
,2
]}

廖富蘋 ^{[1
]}

林健荣 ^{[1
]}

机构：

[1] 华南农业大学动物科学学院

[2] 温州市农业科学研究院

来源：

中国农业科学 | 2009年 / 42卷 / 06期

关键词：

CP7菌; 多粘类芽孢杆菌; 抗菌物质; 分离纯化; 结构分析;

D O I：

暂无

中图分类号：

S476 [生物防治];

学科分类号：

0904 ;

摘要：

【目的】分离和纯化Paenibacillus polymyxaCP7菌抗革兰氏阴性菌的活性组分,并鉴定其结构。【方法】采用固相萃取柱分离、阳离子交换层析、凝胶过滤层析和HPLC层析等技术对活性组分进行分离纯化,通过紫外-可见光谱、圆二色光谱、红外光谱和MALDI-TOF-TOF-MS质谱对所分离组分进行结构鉴定。【结果】从CP7菌发酵液中分离获得抗革兰氏阴性菌的B、C1和C2组分,分子量分别为1155、1169和1191D。并确定了C1组分的分子式为C53H100O13N16,结构由6个L-α,β-二氨基丁酸(DAB)、2个苏氨酸和2个亮氨酸组成,与多粘菌素E1的结构相同。【结论】从CP7菌发酵液中分离获得了3种抗革兰氏阴性菌的小分子多肽。经测试确认C1组分为多粘菌素类E1。推测B组分也是多粘菌素类的1个成员,而C2组分则可能是多粘菌素家族中的一个新成员。

引用

页码：2105 / 2110

页数：6

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