木质纤维素稀酸水解液乙醇发酵的新方法

被引:12
作者
钱名宇
张晶
刘继开
杨秀山
机构
[1] 首都师范大学生命科学学院
关键词
木质纤维素乙醇发酵; 混合菌种; 补料批式发酵; 间隔补料批式发酵;
D O I
暂无
中图分类号
TQ223.122 [];
学科分类号
0817 ;
摘要
为了降低木质纤维素水解液发酵抑制剂对乙醇发酵的负影响,采用混合菌种对木质纤维素稀酸水解液乙醇发酵方式进行了研究。对批式发酵、补料批式发酵和间隔补料批式发酵3种发酵方式进行了比较。实验结果表明,间隔补料批式发酵可以有效地减弱水解液中抑制因子对菌种的影响,乙醇产量明显高于其他两种发酵方式,利用酿酒酵母(Saccaromyces cerevisiae 2.535)和嗜鞣管囊酵母(Pachysolen tannophilis ATCC 32728)混合发酵,乙醇产量最终达到14.4g/L,乙醇产率(Yp/s)为0.47g/g,相当于最大理论产率的92.2%。利用酿酒酵母和重组大肠杆菌混合菌种发酵,乙醇产量达到了14.5g/L。对木质纤维素稀酸水解液采用间隔补料批式乙醇发酵方法,可进一步减少抑制剂对乙醇发酵的影响,使发酵顺利进行。
引用
收藏
页码:618 / 622
页数:5
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