Tat蛋白的PTD区段促进GFP蛋白进入骨髓瘤细胞SP2/0

被引：2

作者：

李志奇

胡小波

杨胜利

龚毅

机构：

[1] 中国科学院上海生命科学研究院生物工程研究中心,中国科学院上海生命科学研究院生物工程研究中心,中国科学院上海生命科学研究院生物工程研究中心,中国科学院上海生命科学研究院生物工程研究中心上海,上海,上海,上海

来源：

生物工程学报 | 2002年 / 05期

关键词：

Tat蛋白; 融合蛋白传递; 骨髓瘤细胞;

D O I：

10.13345/j.cjb.2002.05.029

中图分类号：

Q819 [生物工程应用];

学科分类号：

0836 ; 090102 ;

摘要：

In order to detect the protein delivery mediated by the PTD (protein transduction domain) of TAT Protein, a expression vector, named pT7460-GFP, was constructed by insert the PTD DNA Sequence, followed by a GFP (green fluorescent protein) gene fused in-frame, into the pT7450 vector. The TAT-GFP fusion protein was expressed in the E.coli ER2566. Most of the fusion protein was presented in the inclusion body. The protein was purified by Ni 2+ affinity chromatography under denature conditions, then by a Sepharose Q column to remove urea. The soluble denatured protein was added directly to medium containing the Myeloma Cell SP2/0. It came out that the fusion protein could be detected delivered into the cells under fluorescent microscope in a short time.

引用

页码：645 / 648

页数：4

共 12 条

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