羟基磷灰石结合β-磷酸三钙及海藻酸盐作为牙槽骨修复材料的比较分析

被引：4

作者：

程扬 ^{[1
]}

刘敏 ^{[1
]}

朱忠焰 ^{[2
]}

高莎莎 ^{[3
]}

机构：

[1] 西南医科大学附属口腔医院

[2] 自贡市第一人民医院口腔科

[3] 德阳市口腔医院

来源：

中国组织工程研究 | 2019年 / 23卷 / 30期

关键词：

羟基磷灰石; β-磷酸三钙; 海藻酸盐; 组织工程; 颌骨缺陷; 骨再生; 生物支架材料; 生物相容性;

D O I：

暂无

中图分类号：

R783.1 [牙科材料学];

学科分类号：

100301 [口腔基础医学];

摘要：

背景:牙槽骨修复材料的种类很多,可以分为天然生物材料,人工合成材料和复合材料,各种类型的材料还可以通过化学生物等方法进行合成,其均有各自的优势和不足。目的:比较羟基磷灰石/β-磷酸三钙、纳米碳化羟基磷灰石海藻酸盐及单纯羟基磷灰石3种牙槽骨修复材料的细胞毒性及生物相容性。方法:将羟基磷灰石/β-磷酸三钙浸提液、纳米碳化羟基磷灰石海藻酸盐浸提液、羟基磷灰石浸提液分别与小鼠成骨前细胞、人成骨细胞共培养,XTT实验检测细胞线粒体活性(以单独培养的细胞为对照组),结晶紫分析实验检测细胞毒性(以DMSO培养的细胞为对照组)。取20只Wistar大鼠(购自北京维通利华实验动物有限公司),制备上颌右侧中切牙牙槽骨缺损模型,随机分4组干预:对照组不植入任何材料,其余3组分别植入羟基磷灰石/β-磷酸三钙、纳米碳化羟基磷灰石海藻酸盐及单纯羟基磷灰石材料,植入后7,21,42 d,检测血清中RANKL、骨保护素质量浓度。实验已通过西南医科大学动物伦理委员会审批批准,审批号:IACUC20170315-07。结果与结论:①3组材料浸提液中小鼠成骨前细胞的线粒体活性与对照组比较差异无显著性意义(P> 0.05),3组材料浸提液中人成骨细胞的线粒体活性与对照组比较差异均无显著性意义(P> 0.05);3组材料浸提液中,纳米碳化羟基磷灰石海藻酸盐浸提液中两种细胞的线粒体活性最高;②3组材料浸提液对小鼠成骨前细胞与人成骨细胞的毒性均明显低于对照组(P <0.05);3组材料浸提液中,纳米碳化羟基磷灰石海藻酸盐浸提液对两种细胞的毒性最低;③纳米碳化羟基磷灰石海藻酸盐材料植入后7d的RANKL质量浓度低于21d(P<0.001),植入后42 d的骨保护素质量浓度高于植入后7,21 d(P <0.001);④结果表明相对于羟基磷灰石/β-磷酸三钙与单纯羟基磷灰石材料,纳米碳化羟基磷灰石海藻酸盐材料具有更好的生物相容性。

引用

页码：4804 / 4810

页数：7

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