祛风地黄汤对银屑病模型小鼠皮损的改善作用

被引：4

作者：

夏立君

张彦波

机构：

[1] 河北省衡水市中医医院

来源：

中国药业 | 2021年 / 30卷 / 24期

关键词：

祛风地黄汤; 银屑病; 咪喹莫特; 小鼠; 辅助性T细胞17/调节性T细胞失衡; 作用机制;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理]; R-332 [医用实验动物学];

学科分类号：

100402 [劳动卫生与环境卫生学]; 100806 [中药药理学];

摘要：

目的探讨祛风地黄汤对银屑病模型小鼠皮损的改善作用。方法将60只BALB/c小鼠随机分为空白对照组(等体积0.9%氯化钠溶液)、模型组(等体积0.9%氯化钠溶液)、甲氨蝶呤组(1 mg/kg),以及祛风地黄汤低、中、高剂量组(8.76,17.55,35.10 g/kg),各10只。通过局部涂抹咪喹莫特以复制银屑病小鼠模型。建模成功后,各组小鼠灌胃相应药物或等体积0.9%氯化钠溶液。以银屑病皮损面积及严重程度指数(PASI)评估小鼠皮损情况;以苏木素-伊红(HE)染色观察小鼠皮肤组织病理形态学;以酶联免疫吸附法检测小鼠血清中肿瘤坏死因子-β(TNF-β)、白细胞介素10(IL-10)、白细胞介素17(IL-17)、白细胞介素6(IL-6)水平;以免疫组织化学法检测小鼠皮损组织中增殖细胞核抗原(PCNA)及CD3+的表达水平;以Western blot法检测小鼠皮损组织中叉头样转录因子3 (Foxp3)及孤独核受体(RORγt)蛋白的表达水平。结果与模型组比较,甲氨蝶呤组和祛风地黄汤低、中、高剂量组小鼠皮损组织表皮层厚度均显著减小,真皮层炎性细胞浸润程度均显著减轻;小鼠PASI评分和IL-17,IL-6水平,皮损组织PCNA,CD3+表达水平及RORγt蛋白表达水平均显著降低; IL-10、TNF-β及Foxp3蛋白表达水平均显著升高(P <0.05)。结论祛风地黄汤对模型小鼠银屑病样皮损的改善作用明显,其机制可能与辅助性T细胞17/调节性T细胞失衡有关。

引用

页码：59 / 64

页数：6

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