双酚A对鲫雌激素受体表达和雌二醇水平的影响

被引：6

作者：

李惠云

刘鹏威

魏华

机构：

[1] 上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室

来源：

上海水产大学学报 | 2008年 / 17卷 / 06期

关键词：

内分泌干扰物; 双酚A; α型雌激素受体;

D O I：

暂无

中图分类号：

S917.4 [水产动物学];

学科分类号：

0908 ;

摘要：

运用定量PCR技术和免疫荧光法研究了内分泌干扰物双酚A(BPA)对雌鲫的雌激素效应。以不同浓度的BPA[质量分数分别为1 mg/kg,50 mg/kg,100 mg/kg(注射剂量/鱼体重)]对雌鲫进行腹腔注射,然后分别于24 h和48 h检测肝脏中的α型雌激素受体(ERα)mRNA表达以及血清雌二醇(E2)水平。结果表明1 mg/kg和50 mg/kg BPA处理组ERαmRNA水平与对照组相比在48 h内均没有明显变化;100 mg/kg BPA处理组ERαmRNA水平在注射后24 h显著上调(P<0.05),48 h时仍维持较高水平。1 mg/kg BPA处理组E2水平在24 h显著下降(P<0.05),48 h回升至正常水平;50 mg/kg和100 mg/kg BPA处理组E2水平在24 h显著增加(P<0.01),48 h时仍维持较高水平。结果表明,BPA具有雌激素样效应,高浓度的BPA能够诱导雌鲫肝脏ERαmRNA表达和血清E2水平的上升。

引用

页码：641 / 646

页数：6

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