重组人BDNF对Alzheimer病模型神经元作用的实验研究

被引：3

作者：

刘朝晖

胡海涛

马东亮

许杰华

杨蓬勃

机构：

[1] 西安交通大学医学院人体解剖学教研室

[2] 西安交通大学医学院人体解剖学教研室西安

[3] 西安

来源：

西安交通大学学报(医学版) | 2003年 / 01期

关键词：

Alzheimer病; β淀粉样蛋白; 脑源性神经营养因子; 海马神经元;

D O I：

暂无

中图分类号：

R749.16 [];

学科分类号：

100203 ;

摘要：

目的　探讨重组人脑源性神经营养因子 (rhBDNF)对正常原代培养海马神经元及Alzheimer病 (AD)模型海马神经元的作用。方法　预先加入rhBDNF并将 2 0 μmol·L-1的Aβ2 5～ 3 5作用于培养的海马神经元 ,进行形态学观察、胆碱酯酶 (ACHE)组织化学染色、激光共聚焦显微镜及MTT自动比色微量分析。结果　实验对照组海马神经元存活率降至 5 9.5 % ,与空白对照组有显著性差异 (P <0 .0 1)。rhBDNF实验组海马神经元存活率达到 65 .7%～ 72 .6% ,各实验组与实验对照组有显著性差异 (P <0 .0 5 ) ,且 5 0ng·mL-1为rhBDNF最适浓度。实验组胆碱能神经元数量增多 ,胞体直径及突起长度较对照组明显增大 ,其 [Ca2 +]i 相对稳定。结论　rhBDNF对正常培养海马神经元有营养作用 ,能延长细胞生存时间 ;对AD模型海马神经元有保护作用 ,显著降低细胞死亡率 ,抑制了Aβ对神经元的毒性 ,有助于AD的防治

引用

页码：5 / 8+13 +13

页数：5

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