基于UPLC-MS/MS方法研究马兜铃酸肾病大鼠的血清代谢组学

被引：5

作者：

张莉 ^{[1
]}

孟梅霞 ^{[1
]}

安军民 ^{[1
]}

王昌 ^{[1
]}

畅飞 ^{[1
]}

赵英永 ^{[2
]}

机构：

[1] 西安市第四医院

[2] 西北大学生命科学学院

来源：

药物分析杂志 | 2014年 / 11期

关键词：

马兜铃酸肾病(AAN); 马兜铃酸肾毒性; 代谢组学; 生物标示物鉴定; 鹅去氧胆酸; 胆酸; 牛磺鹅脱氧胆酸; 硫酸对甲酚; 肌酐; 酮基脱氧胆酸; 硫酸吲哚酚; 溶血卵磷脂; 磷脂; 胆汁酸; 氨基酸; 代谢指纹图谱;

D O I：

暂无

中图分类号：

O657.63 [质谱分析]; R692 [肾疾病];

学科分类号：

070302 [分析化学]; 100221 [泌尿外科学];

摘要：

目的:基于超高效液相色谱-四极杆时间飞行高清质谱串联(UPLC-QTOF/HDMS)-代谢组学方法研究马兜铃酸Ⅰ诱导的马兜铃酸肾病(AAN)的代谢指纹图谱。方法:基于UPLC-QTOF/HDMS的代谢组学方法测定AAN大鼠血清,质谱数据采用Marker Lynx软件处理,然后使用主成分分析和正交校正的偏最小二乘判别分析法分析AAN模型组和正常对照组之间的代谢物谱差异,并通过S-plot图选取潜在的生物标示物,通过精确分子量、同位素分布、MSE信息和对照品对照,再通过检索HMDB等数据库对潜在的生物标示物进行鉴定。结果:用代谢组学方法鉴定了10个生物标示物,马兜铃酸Ⅰ上调了鹅去氧胆酸、胆酸、牛磺鹅脱氧胆酸、硫酸对甲酚、肌酐、7-酮基脱氧胆酸、硫酸吲哚酚和12-酮基脱氧胆酸,同时下调了溶血卵磷脂(15∶0)和溶血卵磷脂(17∶0)。结论:马兜铃酸诱导的肾损伤干扰了磷脂、胆汁酸、氨基酸的代谢。基于UPLC-QTOF/HDMS代谢组学法能够应用于马兜铃酸诱导的AAN的研究。

引用

页码：1948 / 1953

页数：6

共 21 条

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