重组酶聚合酶扩增技术:一种新的核酸扩增策略

被引：46

作者：

高威芳

朱鹏

黄海龙

机构：

[1] 宁波大学海洋学院

来源：

中国生物化学与分子生物学报 | 2016年 / 32卷 / 06期

关键词：

重组酶聚合酶扩增; 重组酶聚合酶扩增技术; 等温核酸扩增; 应用;

D O I：

10.13865/j.cnki.cjbmb.2016.06.05

中图分类号：

Q503 [生物化学技术];

学科分类号：

摘要：

重组酶聚合酶扩增(recombinase polymerase amplification,RPA)是近年来兴起的一种等温核酸扩增技术,它比聚合酶链式反应(polymerase chain reaction,PCR)及其它等温扩增技术更快速、便捷、高效。本文将详细介绍RPA这项新颖的技术,并对其在医疗诊断、农业、食品、生物安全等方面的研究及应用进展进行综述。期望这项技术得到更多的关注,使其发展更加完善,将来在更多的领域充分发挥作用,甚至书写核酸检测历史新篇章。

引用

页码：627 / 634

页数：8

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