环介导等温扩增技术的研究进展

被引：13

作者：

张仑

殷幼平

王中康

机构：

[1] 重庆大学生物工程学院重庆市基因功能与调控重点实验室

来源：

贵州农业科学 | 2009年 / 37卷 / 06期

关键词：

环介导等温扩增; 检测; 检验检疫;

D O I：

暂无

中图分类号：

Q503 [生物化学技术];

学科分类号：

摘要：

环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是利用2对特殊设计的引物和一种具有链置换活性的DNA聚合酶,在恒温条件下特异、高效地扩增DNA的新技术,扩增产物为一系列反向重复的靶序列构成的茎环结构和多环花椰菜样结构的DNA片段混合物,电泳图谱呈阶梯式带状分布,反应伴有白色的副产物焦磷酸镁沉淀产生,可通过肉眼定性判断反应结果。LAMP技术已在核酸的科学研究、疾病诊断和动物胚胎性别的鉴定等领域得到了广泛的应用。从LAMP技术的原理、引物组成及反应过程、技术特点、方法延伸和应用等方面对其进行了概述,以期为同类研究提供参考。

引用

页码：21 / 25

页数：5

共 14 条

[1] Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay
Hosaka, Norimitsu
Ndembi, Nicaise
Ishizaki, Azumi
Kageyama, Seiji
Numazaki, Kei
Ichimura, Hiroshi
[J]. JOURNAL OF VIROLOGICAL METHODS, 2009, 157 (02) : 195 - 199
[2] Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B[J] . Masaru Ihira,Akane Ohta,Ken Sugata,Sadao Suga,Yoshizo Asano,Tetsushi Yoshikawa.Journal of Virological Methods . 2008 (1)
[3] Neonatal pertussis presenting as acute bronchiolitis: direct detection of the Bordetella pertussis genome using loop-mediated isothermal amplification[J] . Akari Nakamura,Takashi Sakano,Tetsuo Nakayama,Hiroko Shimoda,Yasuyuki Okada,Ryuzo Hanayama,Katsuhiro Nomoto,Tetsushi Suto,Yoshihisa Kinoshita,Takeki Furue,Hiroaki Ono,Toshiyuki Ohta.European Journal of Pediatrics . 2009 (3)
[4] Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method
Ihira, Masaru
Akimoto, Shiho
Miyake, Fumi
Fujita, Ayano
Sugata, Ken
Suga, Sadao
Ohashi, Masahiro
Nishimura, Naoko
Ozaki, Takao
Asano, Yoshizo
Yoshikawa, Tetsushi
[J]. JOURNAL OF CLINICAL VIROLOGY, 2007, 39 (01) : 22 - 26
[5] Rapid detection of Epstein–Barr virus DNA by loop-mediated isothermal amplification method[J] . Seiko Iwata,Yukiko Shibata,Jun-ichi Kawada,Shinya Hara,Yukihiro Nishiyama,Tsuneo Morishima,Masaru Ihira,Tetsushi Yoshikawa,Yoshizo Asano,Hiroshi Kimura.Journal of Clinical Virology . 2006 (2)
[6] Real-time quantitative LAMP (loop-mediated isothermal amplification of DNA) as a simple method for monitoring ammonia-oxidizing bacteria
Aoi, Yoshiteru
Hosogai, Mariko
Tsuneda, Satoshi
[J]. JOURNAL OF BIOTECHNOLOGY, 2006, 125 (04) : 484 - 491
[7] Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus
Dukes, J. P.
King, D. P.
Alexandersen, S.
[J]. ARCHIVES OF VIROLOGY, 2006, 151 (06) : 1093 - 1106
[8] Loop-mediated isothermal amplification for the rapid detection of Salmonella
Hara-Kudo, Y
Yoshino, M
Kojima, T
Ikedo, M
[J]. FEMS MICROBIOLOGY LETTERS, 2005, 253 (01) : 155 - 161
[9] Rapid sexing of bovine preimplantation embryos using loop-mediated isothermal amplification
Hirayama, H
Kageyama, S
Moriyasu, S
Sawai, K
Onoe, S
Takahashi, Y
Katagiri, S
Toen, K
Watanabe, K
Notomi, T
Yamashina, H
Matsuzaki, S
Minamihashi, A
[J]. THERIOGENOLOGY, 2004, 62 (05) : 887 - 896
[10] Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
Endo, S
Komori, T
Ricci, G
Sano, A
Yokoyama, K
Ohori, A
Kamei, K
Franco, M
Miyaji, M
Nishimura, K
[J]. FEMS MICROBIOLOGY LETTERS, 2004, 234 (01) : 93 - 97

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