甘蓝型油菜异胡豆苷合成酶(Strictosidine synthase)cDNA的克隆与分析

被引：3

作者：

王敏培

周云涛

王茂林

赵云

机构：

[1] 四川大学生命科学学院

来源：

四川大学学报(自然科学版) | 2008年 / 05期

关键词：

异胡豆苷合成酶; 无花瓣突变体; RACE-PCR; 甘蓝型油菜;

D O I：

暂无

中图分类号：

S565.4 [油菜籽（芸薹）];

学科分类号：

0901 ;

摘要：

以无花瓣与正常甘蓝型油菜幼嫩花蕾(长度小于0.2mm)建立的差异表达文库中发现的异胡豆苷合成酶基因(STR)的一个cDNA片段为基础,利用RACE方法获得了甘蓝型油菜的STR基因cDNA全序列BnSTR.序列分析表明BnSTR全长1408bp,开放阅读框从第20个碱基到第1291个碱基,推导的蛋白序列与Arabidopsis thaliana,Lycopersicon esculentum,Oryzasativa,Rauvolfia manni,Catharanthus roseus和Ophiorrhiza pumila中STR的同源性分别是91,41.8,36.2,31.2,29.2和28.4%.表达结果显示,与野生型相比,油菜BnSTR在无花瓣突变型幼嫩花蕾中表达量明显下降,BnSTR可能与花瓣的发育有关.

引用

页码：1277 / 1280

页数：4

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