鸡眼草根瘤菌的16S rDNA全序列分析

Based on the previous studies on numerical taxonomy, SDS\|PAGE of whole\|cell protein and DNA hybridization, the rhizobial strains isolated from \%Kummerowia\% sp. in semi\|arid area of North\|west constituted a new subgroup, the 16S rDNA sequence of representative strain SH714 were tested. The unrooted phylogenetic tree was produced. In this tree, the strain SH714 with \%Sinorhizobium xinjiangensis, S. fredii, S. meliloti, S. medicae, S. saheli\% and \%S. teranga\% constituted a branch of \%Sinorhizobium.\% Within this branch, the similarity valuse of 16S rDNA sequence between strain SH714 and \%S. xinjiangesis, S. fredii, S. meliloti, S. medicae, S. saheli\% and \%S. teranga\% were 97.4%, 97.5%, 96.8%, 96.7%, 97.2% and 95.6% respectively, the values were more than 95%, this indicated that these known species should belong to the same genus. The values of DNA homology between type strains of these species were less than 70%. Thus, the strain SH714 represented a new rhizobial species, and there were some diversity between SH714 and known rhizobial species in phenotypic feature and composition of protien.