偏侧帕金森大鼠模型各个发病时期多巴胺能系统的动态监测

被引：3

作者：

曹非

陈涵

张潇潇

孙圣刚

机构：

[1] 华中科技大学同济医学院附属协和医院神经内科

来源：

脑与神经疾病杂志 | 2012年 / 20卷 / 05期

关键词：

帕金森病; 6-羟多巴胺; 左旋多巴; 多巴胺;

D O I：

暂无

中图分类号：

R742.5 [震颤麻痹综合征];

学科分类号：

摘要：

目的动态监测帕金森大鼠不同发病阶段多巴胺能系统的改变,掌握其变化规律和各指标间的量化关系,为进一步实验提供动物治疗模型。方法偏侧两点注射法制作帕金森病大鼠模型,模型成功两周后给予小剂量左旋多巴治疗一周。每周检测纹状体多巴胺(DA)含量,黑质酪氨酸羟化酶(TH)染色和尼氏染色,观察行为学改变。结果一周后即发病的代偿期,行为学开始出现向健侧的旋转;两周后即失代偿期,旋转次数加重至最高峰。前两周多巴胺含量分别较正常对照侧减少16.7%和80%,TH阳性细胞分别下降47.97%和93.28%,尼氏细胞下降31.4%和46.4%.给予多巴胺治疗一周后,旋转圈数及TH染色、尼氏染色没有显著性改变,多巴胺含量明显增加到58.3%。结论短期小剂量给予左旋多巴治疗晚期PD仅能提高多巴胺含量。

引用

页码：360 / 364

页数：5

共 11 条

[1]

Automated gait analysis in bilateral Parkinsonian rats and the role of L-DOPA therapy. Westin JE,Janssen ML,Sager TN,et al. Behavioural Brain Research . 2012

[2]

Effect of adenosine A2A receptor antagonists and L-DOPA on hydroxyl radical, glutamate and dopamine in the striatum of 6-OHDA-treated rats. K Go?embiowska,A Dziubina. Neurotox Res . 2012

[3]

Comparison of the behavioural and histologicalcharacteristics of the 6-OHDA andα-synuclein rat models ofParkinson’’s disease. Decressac M.et al. Experimental Neurology . 2012

[4]

Differential modulation by nicotine of substantia nigra versus ventral tegmental area dopamine neurons. J. R. Keath,M. P. Iacoviello,L. E. Barrett, et al. Journal de Neuroradiologie . 2007

[5]

Unilateral nigrostriatal 6-hydroxydopaminelesions in mice I:motor impairments identify extent of dopamine de-pletion at three different lesion sites. Heuer A,Smith GA,et al. Behavioural Brain Research . 2012

[6]

Acute L-DOPA Effect on Hydroxyl Radical-andDOPAC-Levelsin Striatal Microdialysates of Parkinsonian Rats. Nowak P,et al. Neurotox Res . 2010

[7]

Pentobarbital inhibits l-DOPA-induced dopamineincreases in the rat striatum:An in vivo microdialysis study. Yushi U,et al. Brain Research Bulletin . 2006

[8]

Aging, plasticity and environmental enrichment: Structural changes and neurotransmitter dynamics in several areas of the brain[J] . Francisco Mora,Gregorio Segovia,Alberto del Arco. &nbspBrain Research Reviews . 2007 (1)

[9]

Histological,be-havioural and neurochemical evaluation of medialforebrain bundle and striatal 6-OHDA lesions as ratmodels of Parkinson’’s disease. Yuan H,Sarre S,Ebinger G,et al. Journal of Neuroscience Methods . 2005

[10]

L-DOPA-induced dopamine efflux in the striatum and the substantia nigra in a rat model of Parkinson’s disease: temporal and quantitative relationship to the expression of dyskinesia. HS Lindgren,DR Andersson,S Lagerkvist,H Nissbrandt,MA Cenci. Journal of Neurochemistry . 2010

← 1 2 →