包含体蛋白质的复性研究进展

被引:20
作者
宁云山
李妍
王小宁
机构
[1] 第一军医大学分子免疫研究所
[2] 第一军医大学分子免疫研究所 广州
[3] 广州
关键词
包含体; 聚集; 复性;
D O I
暂无
中图分类号
Q591.2 [蛋白质代谢];
学科分类号
071010 ; 081704 ;
摘要
包含体的形成是异源蛋白质在大肠杆菌中高效表达的必然结果 ,也是目前产生重组蛋白质最有效的方法之一。不可溶、无生物活性的包含体必须经过变性、复性才能获得天然结构 ,完成特定的生物学功能。聚集是造成重组蛋白质复性产率低下的主要因素 ,因此理解蛋白质聚集机制 ,减少和防止聚集的发生是建立高效、高产率复性方法的关键。分子伴侣、低分子量添加物等在复性过程中的应用及新的复性方法的建立都大大提高了重组蛋白质复性产率
引用
收藏
页码:237 / 240
页数:4
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