厚朴酚调控人小细胞肺癌H446细胞凋亡的机制研究

被引：3

作者：

董秀 ^{[1
,2
]}

于丹 ^{[2
]}

王淳 ^{[2
]}

王晓波 ^{[2
]}

王梅 ^{[2
]}

李建春 ^{[1
]}

机构：

[1] 沈阳药科大学

[2] 辽宁中医药大学

来源：

辽宁中医杂志 | 2012年 / 39卷 / 08期

关键词：

厚朴酚; 小细胞肺癌; 细胞凋亡;

D O I：

10.13192/j.ljtcm.2012.08.10.dongx.063

中图分类号：

R734.2 [肺肿瘤]; R285.5 [中药实验药理];

学科分类号：

100214 ; 1008 ;

摘要：

目的:探讨厚朴酚对人小细胞肺癌H446细胞增殖和凋亡的影响。方法:体外培养人小细胞肺癌H446细胞,经不同浓度和不同时间的厚朴酚作用后,应用MTT法检测厚朴酚对H446细胞增殖的影响;采用流式细胞仪罗丹明123染色法检测线粒体膜电位的改变;蛋白印迹方法检测凋亡相关蛋白的表达。结果:厚朴酚可呈时间剂量依赖性抑制H446细胞增殖,诱导线粒体膜电位的下降,免疫印迹结果显示Bcl-2的表达降低,Bax、Cyto.c和活化pro-caspase 9的表达增加。结论:厚朴酚可显著抑制H446细胞增殖,介导线粒体途径的细胞凋亡。

引用

页码：1443 / 1445

页数：3

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