感染萧氏松茎象的金龟子绿僵菌菌株的初步筛选

A total of 12 strains of Metarhizium anisopliae Sorokin were tested for pathogenicity to Hylobitelus xiaoi,and a superior strain was used to infect trials in a forest.The results indicated that the strain Ma1291-2 had strong pathogenicity to H.xiaoi after treatment for 30 d by dipping inoculation with （1.5±0.5）×108 conidia·mL-1.The correct mortality,the muscardine cadaver rate and the conidia content individual of H.xiaoi were respectively 70.59%,70.81% and （1.90±0.34）×108 conidia individual,and the quickest median lethal times （LT_ 50 ） were 13.89 d.The median lethal concentration （LC_ 50 ） was 1.135 3 ×106 conidia·mL-1.By infecting trials to H.xiaoi in a forest with the strain Ma1291-2,mortality of the treatment was extremely significant higher than the control,the correct mortality,the muscardine cadaver rate and the conidia content individual of H.xiaoi were 57.85%±1.19%,55.43%±2.90% and （1.12±0.41）×108 conidia individual after 30 d,respectively,and the median lethal times （LT_ 50 ） was 16.56 d.The results suggest that the strain of Ma1291-2 has great application value.