von Willebrand因子胶原结合试验的建立及其临床应用

被引：3

作者：

施文瑜

王迎春

白霞

陆德炎

阮长耿

机构：

[1] 南通医学院附属医院血液科

[2] 江苏省血液研究所

来源：

中华检验医学杂志 | 2003年 / 04期

关键词：

von Willebrand因子; 酶联免疫吸附试验; 胶原结合试验;

D O I：

暂无

中图分类号：

R446.6 [免疫学检验];

学科分类号：

摘要：

目的　建立了一种新的检测vonWillebrand因子 (vWF)功能的方法。　方法　用ELISA法检测血浆中vWF与胶原的结合能力。　结果　该法敏感性为 0 0 0 1U/ml,批内和批间变异为3 34%和 6 70 %。 2 0名正常人血浆的胶原结合试验 (vWF :CBA)值为 (90 2 4± 2 2 87) %。 5 4例初步诊断为血管性血友病 (vWD)患者vWF :CBA值为 (31 94± 2 7 36 ) % ,10例 1型vWD为 (35 2 2± 2 0 0 2 ) % ,10例 2A型vWD为 (8 74± 6 38) % ,6例 3型vWD为 (0 70± 0 5 8) % ,以上各组值均低于正常值 (P <0 0 1) ;2A型vWD患者vWF :CBA值小于 1型vWD(P <0 0 1)。　结论　vWF胶原结合试验是一项简单易行、准确反映vWF功能的实验 ,为研究血管性血友病 (vWD)和血栓性疾病提供了一种新的方法 ,可向临床推广。

引用

页码：7 / 10

页数：4

共 15 条

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