淫羊藿苷促进软骨细胞体外3-D培养下生成软骨

被引：11

作者：

王鹏珍 ^{[1
]}

万超 ^{[2
]}

机构：

[1] 暨南大学生命科学与技术学院

[2] 香港中文大学再生医学教育部重点实验室

来源：

中国老年学杂志 | 2015年 / 35卷 / 10期

关键词：

淫羊藿苷; 软骨细胞; 胶原蛋白; 蛋白聚糖; 羟脯氨酸;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

目的探究淫羊藿苷(ICA)对软骨细胞在体外3-D培养条件下的作用。方法将胎鼠原代软骨细胞分成对照组和ICA浓度梯度组,通过MTT法、阿尔新蓝染色、酶联免疫吸附法,确定出ICA最有效浓度为10-6mol/L,并将此浓度用于后续软骨生成实验。软骨细胞接种于Gelfoam上,形成复合块,分为对照组和ICA组,进行形态学和免疫组化以及Real-time PCR分析。结果细胞培养14 d时,ICA能显著促进胶原蛋白和蛋白聚糖分泌。此外,利用Gelfoam进行软骨细胞3-D培养,ICA促使软骨细胞中蛋白聚糖、胶原蛋白(collagen)Ⅱ,HIF-1α和Sox9在mRNA和蛋白水平表达均显著上调,且与对照组相比,差异均有统计学意义(P<0.05)。结论淫羊藿苷可以促进软骨细胞在体外3-D培养条件下生成软骨。

引用

收藏

页码：2597 / 2600

页数：4

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