RAPD标记在鹅掌楸属中的遗传研究

Fourteen 10 mer primers were used for RAPD (Random amplified polymorphic DNA) analysis across a fullsib family, including 2 parents and 18 sibs, of Liriodendron chinense×L. Tulipifera. Of the total 57 fragments amplified, 20 (35 09%) were polymorphic between the 2 parents and 28 (49 12%) segregants in the sibs, indicting a little lower of polymorphism and heterozygosity at RAPD loci in the 2 species, compared with other tree species studied. Of the 20 polymorphic loci, 4 (appeared in the male parent) did not segregate in the progeny, indicating the genotype in these loci were AA ( α =0 5 18 ), so, may be used as paternal markers for paternal identification. Fourteen parental polymorphic loci (24 56%) segregated 1∶1 in the progeny as expected, and 12 parental monomorphic loci (21 05%) segregated 3∶1 as expected, showing a Mendelian dominant inheritance fashion for an allele. Two parental monomorphic loci (3 51%) were detected to segregate in aberration from the expected ratios (3∶1).