颅内动脉瘤患者血清蛋白组变化的研究

被引：5

作者：

朱永华 ^{[1
]}

刘炜 ^{[2
]}

石忠松 ^{[1
]}

刘少军 ^{[2
]}

李明昌 ^{[3
]}

黎明涛 ^{[2
]}

潘伟生 ^{[4
]}

黄正松 ^{[1
]}

机构：

[1] 中山大学附属第一医院神经外科

[2] 中山大学基础医学院蛋白质组学实验室

[3] 广州医学院第二附属医院神经外科

[4] 香港中文大学威尔斯亲王医院外科学系

来源：

中山大学学报(医学科学版) | 2006年 / 06期

关键词：

双向胶内差异凝胶电泳; 图像分析; 血清蛋白; 颅内动脉瘤; 基质辅助激光解析离子化飞行时间质谱; 结合珠蛋白;

D O I：

暂无

中图分类号：

R651.1 [颅脑];

学科分类号：

1002 ; 100210 ;

摘要：

【目的】寻找颅内动脉瘤患者血清中表达差异的蛋白。【方法】采集12例血清蛋白样本(颅内动脉瘤患者4例、脑挫裂伤患者4例及正常成人本4例)用不同的CyDye染料交叉标记后依次进行双向胶内差异凝胶电泳(2-DDIGE)、图像分析及基质辅助激光解析离子化飞行时间质谱(MALDI-TOF-MS)鉴定。【结果】发现胶号为1119的蛋白质点,在颅内动脉瘤患者血清中比正常成人组表达升高1.82倍(P<0.05),而在脑挫裂伤患者血清中与正常成人的表达差异没有统计学意义(P>0.05)。该蛋白点经质谱鉴定为结合珠蛋白。【结论】结合珠蛋白在颅内动脉瘤患者血清中表达升高,可能参与了颅内动脉瘤的形成及扩张。

引用

页码：652 / 656

页数：5

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