α-硫辛酸通过激活ERK1/2减轻葡萄糖/葡萄糖氧化酶所致心肌细胞损伤

被引:1
作者
张阳阳
姚玉珍
李荣荣
刘莉
机构
[1] 南京医科大学第一附属医院老年医学科
关键词
α-硫辛酸; 葡萄糖/葡萄糖氧化酶; 心肌细胞; 胞外信号调节激酶;
D O I
暂无
中图分类号
R587.2 [糖尿病性昏迷及其他并发症];
学科分类号
1002 ; 100201 ;
摘要
目的探讨α-硫辛酸(LA)对葡萄糖/葡萄糖氧化酶(DG/GO)所致心肌细胞损伤的保护机制,着重探讨ERK1/2在其中的作用。方法将培养的大鼠H9c2心肌细胞分为如下几组:对照组、LA组、DG/GO组、LA+DG/GO组、PD+LA组、PD+LA+DG/GO组,3h后收集细胞测定ERK1/2磷酸化激活、24h测定细胞存活率。结果 LA显著提高ERK1/2磷酸化水平;PD98059完全阻断LA对ERK1/2的激活作用;以PD98059阻断LA对ERK1/2的激活后,完全去除LA对DG/GO所致细胞损伤的保护作用。结论 LA对DG/GO致心肌细胞损伤有保护性作用,是通过激活ERK1/2依赖性信号通路介导的。
引用
收藏
页码:547 / 550
页数:4
相关论文
共 6 条
[1]   α-硫辛酸治疗2型糖尿病周围神经病变22例疗效观察 [J].
谷雪静 ;
王燕 ;
樊海英 .
实用老年医学, 2012, 26 (01) :47-49
[2]  
Modulation of the JAK/ERK/STAT signaling in melanocortin-induced inhibition of local and systemic responses to myocardial ischemia/reperfusion[J] . Alessandra Ottani,Maria Galantucci,Ettore Ardimento,Laura Neri,Fabrizio Canalini,Anita Calevro,Davide Zaffe,Ettore Novellino,Paolo Grieco,Daniela Giuliani,Salvatore Guarini. Pharmacological Research . 2013
[3]   Pigment epithelium-derived factor inhibits oxidative stress-induced cell death by activation of extracellular signal-regulated kinases in cultured retinal pigment epithelial cells [J].
Tsao, Yeou-Ping ;
Ho, Tsung-Chuan ;
Chen, Show-Li ;
Cheng, Huey-Chuan .
LIFE SCIENCES, 2006, 79 (06) :545-550
[4]   Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy [J].
Cai, Lu ;
Wang, Yuehui ;
Zhou, Guihua ;
Chen, Teresa ;
Song, Ye ;
Li, Xiaokun ;
Kang, Y. James .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2006, 48 (08) :1688-1697
[5]  
Neuroprotective effects of α-lipoic acid and its enantiomers demonstrated in rodent models of focal cerebral ischemia[J] . P. Wolz,J. Krieglstein. Neuropharmacology . 1996 (3)
[6]  
Treatment of symptomatic diabetic peripheral neuropathy with the anti-oxidant α-lipoic acid[J] . D. Ziegler,M. Hanefeld,K. J. Ruhnau,H. P. Meier,M. Lobisch,K. Schütte,F. A. Gries. Diabetologia . 1995 (12)