HIV-1耐药性基因型检测法在临床治疗随访中的应用

被引：3

作者：

徐军强

魏飞力

梅珊

曹韵贞

机构：

[1] 卫生部艾滋病预防与控制中心

[2] 卫生部艾滋病预防与控制中心（北京市）

来源：

中国艾滋病性病 | 2002年 / 02期

关键词：

HIV-1; 耐药性; 蛋白酶基因; 逆转录酶基因; RT-PCR; 序列测定;

D O I：

10.13419/j.cnki.aids.2002.02.003

中图分类号：

R512.91 [获得性免疫缺陷综合征（AIDS艾滋病）];

学科分类号：

100401 ;

摘要：

目的探索HIV-1耐药性基因型检测法在高效抗逆转录病毒疗法（HAART）治疗组中监测HIV-1耐药性病毒株的临床应用。方法从接受HAART的HIV-1感染者血浆中抽提病毒RNA,采用套式RT-PCR方法扩增HIV-1的PR和RT基因片段,并对扩增片段进行序列测定和分析。结果HIV-1耐药性基因型检测法能够从血浆病毒载量在 1000拷贝/ml以上的样本中得到扩增产物。在 16例接受 HAART的 HIV-1感染者中,发现 1例感染者 DP31的 PR和 RT基因出现了突变,这些突变为L63P、T215F、K219Q和M184V。所有突变均与公开报道的结果一致,并被证明与HIV-1的耐药性有关。另外,DP31在接受HAART前已出现T215F、K219Q的耐药性突变,究其原因,还有待进一步研究。结论HIV-1耐药性基因型检测法能有效地监测接受HAART的HIV-1感染者血浆中耐药性病毒株的存在。该方法提供的结果准确、可靠,并且为临床医生评价HAART效果,合理选择和及时优化药物组合方案提供了可靠的依据。

引用

页码：74 / 77

页数：4

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