曲古菌素A对脂多糖致急性肺损伤小鼠的保护作用观察

被引：11

作者：

雷鸣

倪云峰

李小飞

张志培

刘涛

程庆书

机构：

[1] 第四军医大学唐都医院胸腔外科

来源：

解放军医学杂志 | 2012年 / 37卷 / 04期

关键词：

曲古菌素A; 肺损伤,急性; 脂多糖类; 细胞因子类;

D O I：

暂无

中图分类号：

R563.8 [呼吸衰竭];

学科分类号：

100201 [内科学];

摘要：

目的探讨曲古菌素A(TSA)对脂多糖(LPS)所致急性肺损伤小鼠的保护作用及其机制。方法健康雄性BALB/c小鼠60只,随机分为空白对照组、TSA组(灌胃给予TSA 1mg/kg)、LPS组(气管内给予LPS 1mg/kg)及TSA+LPS组(气管内给予LPS前1h灌胃给予TSA),每组15只。分别于处理后1、3、6、12、24h获取各组小鼠支气管肺泡灌洗液(BALF),采用ELISA法检测其中TNF-α和IL-1β的浓度,并取肺组织测定肺干湿重比,HE染色后行病理组织学观察,ELISA法检测组织匀浆中髓过氧化物酶(MPO)活性及一氧化氮(NO)浓度。结果与空白对照组及TSA组比较,LPS组小鼠肺组织可见明显的炎性细胞浸润等急性肺损伤病理学征象,肺组织湿干重比、MPO活性、NO浓度及BALF中TNF-α、IL-1β浓度均明显升高(P<0.05)。与LPS组比较,TSA+LPS组上述改变均明显受抑,肺组织损伤减轻,组间比较差异有统计学意义(P<0.05)。结论 TSA对LPS诱导的急性肺损伤有保护作用,可能与其抑制了炎性细胞因子的生成有关。

引用

页码：283 / 287

页数：5

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