石蜡包埋组织实时荧光定量PCR技术在结核病诊断中的应用

被引：11

作者：

娄丽萍 ^{[1
]}

张文迪 ^{[2
]}

机构：

[1] 华中科技大学同济医学院附属同济医院病理研究所

[2] 华中科技大学同济医学院附属同济医院儿科

来源：

锦州医科大学学报 | 2019年 / 40卷 / 03期

关键词：

石蜡包埋组织; 结核病; 实时荧光定量PCR; 抗酸染色;

D O I：

10.13847/j.cnki.lnmu.2019.03.023

中图分类号：

R52 [结核病];

学科分类号：

1002 ; 100201 ;

摘要：

目的探讨石蜡包埋组织实时荧光定量PCR (RT-q PCR)检测结核分枝杆菌DNA在结核病诊断中的应用价值。方法收集58例临床确诊为结核病与40例非结核肉芽肿性疾病的石蜡包埋组织标本,应用RT-q PCR检测标本中结核分枝杆菌DNA,同时进行Ziehl-Neelsen抗酸染色,比较RT-q PCR与抗酸染色检测结果的差异。结果 58例结核病例中通过RT-q PCR检测有56例为阳性,灵敏度为96. 6%(56/58);通过抗酸染色检测有16例为阳性,灵敏度为27. 6%(16/58),两者灵敏度差异有统计学意义(P<0. 001)。而40例非结核性肉芽肿病例通过RT-q PCR检测有39例为阴性,特异度为97. 5%(39/40),通过抗酸染色检测有36例为阴性,特异度为90. 0%(36/40),两者特异度差异无统计学意义(P=0. 359)。结论相对结核抗酸染色,RT-q PCR灵敏度较高,是石蜡包埋组织快速检测结核分枝杆菌DNA的有效工具,是快速诊断结核病的重要手段。

引用

页码：74 / 76+119 +119

页数：4

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