鱼肉中喹诺酮类药物残留检测前处理方法的研究

被引：10

作者：

李慧芳 ^{[1
]}

殷军港 ^{[2
]}

刘永明 ^{[1
]}

机构：

[1] 烟台大学化学化工学院

[2] 烟台大学生命科学学院

来源：

中国渔业质量与标准 | 2012年 / 2卷 / 01期

关键词：

QuEChERS; 鱼肉; 氟喹诺酮; 高效液相色谱;

D O I：

暂无

中图分类号：

S948 [水产药物学、药理学];

学科分类号：

0906 ;

摘要：

利用改进的QuEChERS前处理方法提取鱼肉中依诺沙星(EO)、诺氟沙星(NRF)、盐酸环丙沙星(CPFHCl)和恩诺沙星(ERF)4种氟喹诺酮类药物。采用90%乙腈酸溶液作为提取剂,结合分散固相萃取(DSPE)净化,以PSA固体颗粒作吸附剂,利用高效液相色谱(HPLC)进行检测。4种药物回收率可达80%～96%,相对标准偏差均小于6%。结果证明此方法能够满足鱼肉中氟喹诺酮类兽药残留的简便、快速、高效检测。

引用

页码：62 / 66

页数：5

共 8 条

[1]

Simultaneous determination of quinolones in fish by liquid chromatography coupled with fluorescence detection: Comparison of sub‐2?μm particles and conventional C18 columns[J] . HongZhang,SiChen,YanbinLu,ZhiyuanDai.J. Sep. Science . 2010 (13)

[2] Determination of fluoroquinolone residues in poultry muscle in Portugal [J].

Pena, A. ;

Silva, L. J. G. ;

Pereira, A. ;

Meisel, L. ;

Lino, C. M. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 397 (06) :2615-2621

[3]

The development and validation of a multiclass liquid chromatography tandem mass spectrometry (LC–MS/MS) procedure for the determination of veterinary drug residues in animal tissue using a QuEChERS (QUick, Easy, CHeap, Effective, Rugged and Safe) approach[J] . George Stubbings,Timothy Bigwood.Analytica Chimica Acta . 2009 (1)

[4]

Multi-residue determination of seven quinolones antibiotics in gilthead seabream using liquid chromatography–tandem mass spectrometry[J] . Journal of Chromatography A . 2008 (2)

[5]

Confirmatory and quantitative analysis using experimental design for the extraction and liquid chromatography–UV, liquid chromatography–mass spectrometry and liquid chromatography–mass spectrometry/mass spectrometry determination of quinolones in turkey muscle[J] . M. Clemente,M.P. Hermo,D. Barrón,J. Barbosa.Journal of Chromatography A . 2006 (2)

[6] Determination of quinolones and fluoroquinolones in fish tissue and seafood by high-performance liquid chromatography with electrospray ionisation tandem mass spectrometric detection [J].

Johnston, L ;

Mackay, L ;

Croft, M .

JOURNAL OF CHROMATOGRAPHY A, 2002, 982 (01) :97-109

[7] Validation of an analytical procedure for the determination of the fluoroquinolone ofloxacin in chicken tissues [J].

Maraschiello, C ;

Cusidó, E ;

Abellán, M ;

Vilageliu, J .

JOURNAL OF CHROMATOGRAPHY B, 2001, 754 (02) :311-318

[8]

Quantitation of nine quinolones in chicken tissues by high-performance liquid chromatography with fluorescence detection[J] . J.C Yorke,P Froc.Journal of Chromatography A . 2000 (1)

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