鞘内注射胍丁胺对骨癌痛大鼠痛觉及脊髓背角NMDA受体1表达的影响

被引：1

作者：

张斌

张涛

杨承祥

仲吉英

机构：

[1] 佛山市第一人民医院麻醉科

来源：

临床麻醉学杂志 | 2012年 / 28卷 / 08期

关键词：

骨癌痛; 胍丁胺; NMDA受体1;

D O I：

暂无

中图分类号：

R738 [运动系肿瘤];

学科分类号：

100214 ;

摘要：

目的观察鞘内注射胍丁胺对骨癌痛大鼠痛觉及脊髓背角NMDA受体1(NR1)表达的影响。方法成年雌性Wistar大鼠,体重180～220g,假模型组(A组,n=14)接种PBS液,骨癌痛模型制作成功大鼠随机分为三组,其中B组(n=14)不行鞘内注射,另两组分别鞘内注射生理盐水10μl(C组,n=14)和胍丁胺160mg/kg(D组,n=14)。用vonFrey丝测定大鼠机械缩足反射阈值(MWT),于鞘内注射后120min用Westernblot方法测定脊髓背角NR1蛋白的表达。结果建模7d后B、C和D组大鼠MWT明显低于A组(P<0.05),鞘内注射胍丁胺后D组MWT显著高于B、C组(P<0.05)。鞘内给药120min后B、C、D组NR1蛋白的相对表达量明显高于A组(P<0.05),D组明显低于B、C组(P<0.05)。结论鞘内注射胍丁胺可通过抑制大鼠脊髓背角NR1的表达减轻胃癌痛。

引用

页码：795 / 797

页数：3

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