利用高通量测序对三江平原小叶章湿地土壤细菌多样性的研究

被引：73

作者：

隋心 ^{[1
,2
,3
,4
]}

张荣涛 ^{[1
,2
,3
]}

钟海秀 ^{[1
,2
,3
]}

许楠 ^{[1
,2
,3
]}

王继丰 ^{[1
,2
,3
]}

刘应竹 ^{[1
,2
,3
]}

袁海峰 ^{[1
,2
,3
]}

倪红伟 ^{[1
,2
,3
]}

机构：

[1] 黑龙江省科学院自然与生态研究所

[2] 湿地与生态保育国家地方联合工程实验室

[3] 黑龙江省湿地与恢复生态学重点实验室

[4] 东北林业大学

来源：

土壤 | 2015年 / 47卷 / 05期

关键词：

16S rDNA; 湿地; Miseq;

D O I：

10.13758/j.cnki.tr.2015.05.017

中图分类号：

S154.3 [土壤微生物学];

学科分类号：

090301 [土壤学];

摘要：

利用黑龙江省科学院自然与生态研究所三江平原野外实验研究站内3个不同小叶章生态类型湿地土壤样品,直接提取土壤微生物总DNA,应用Miseq测序技术对16S rDNA进行序列测定和分析。结果表明:不同小叶章湿地土壤细菌群落结构发生了显著变化,土壤细菌多样性和丰富度随着土壤含水率的增加而降低。草甸化湿地和沼泽化草甸湿地优势种群为酸杆菌,变形菌次之;沼泽化湿地优势种群为变形菌,酸杆菌次之。土壤含水率的增加减少了酸杆菌的分布,而增加了变形菌的分布。16S rDNAheatmap分析则表明,湿地水位的变化对酸杆菌和变形菌的群落结构影响最大。

引用

页码：919 / 925

页数：7

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