甲状腺乳头状癌PI3K/Akt信号转导通路mRNA和蛋白表达及其生物学意义的探讨

被引：4

作者：

于世鹏

伊鹏飞

孙琳

王娜

机构：

[1] 济宁医学院附属医院内分泌科

来源：

中华肿瘤防治杂志 | 2012年 / 19卷 / 13期

关键词：

甲状腺肿瘤; PI3K; Akt;

D O I：

10.16073/j.cnki.cjcpt.2012.13.019

中图分类号：

R736.1 [甲状腺肿瘤];

学科分类号：

摘要：

目的:探讨甲状腺乳头状癌(PTC)中PI3K mRNA、Akt mRNA、PI3K和Akt的表达与意义。方法:采用逆转录聚合酶链反应(RT-PCR)测定组织中PI3KmRNA和Akt mRNA的表达;应用免疫组化法测定组织中PI3K和Akt蛋白的表达。结果:PTC中PI3KmRNA表达量(90.3±5.06)和Akt mRNA的表达量(78.13±5.5)高于癌旁正常甲状腺组织(NAT)表达量37.74±10.63和14.82±5.63,P<0.05。免疫组化结果显示,甲状腺乳头状癌PI3K蛋白表达阳性率73.33%、Akt蛋白表达阳性率(83.33%)明显高于NAT组织蛋白表达阳性率33.33%和16.67%,P<0.05。PTC中PI3K和Akt蛋白表达与包膜浸润及淋巴结转移相关,P<0.05。结论:PTC中存在PI3K/Akt信号传导通路的异常激活,信号传导途径的激活水平与PTC浸润和局部转移有关。

引用

页码：993 / 995

页数：3

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