半边旗抗肿瘤有效成分对HL-60细胞DNA拓扑异构酶活性及其细胞周期的影响(英文)

被引：11

作者：

李金华

何承伟

梁念慈

莫丽儿

张晓

机构：

[1] 广东医学院医用生化研究所

[2] 广东医学院化学教研室!湛江

[3] 中国

来源：

Acta Pharmacologica Sinica | 1999年 / 06期

关键词：

半边旗; 二萜; DNA拓扑异构酶; 细胞周期; 金雀异黄素; HL-60细胞; 药物协同作用; 抗肿瘤药;

D O I：

暂无

中图分类号：

R96 [药理学];

学科分类号：

100602 ; 100706 ;

摘要：

研究半边旗（Ｐｔｅｒｉｓｓｅｍｉｐｉｎｎａｔａ　Ｌ，ＰｓＬ）有效成分５Ｆ，６Ｆ，Ａ对ＨＬ－６０细胞ＤＮＡ拓扑异构酶活性和细胞周期的影响．方法：应用ｐＢＲ３２２质粒ＤＮＡ作为底物测定酶的活性；细胞周期用流式细胞仪测定；应用噻唑蓝（ＭＩＴ）法测定药物对细胞生长的抑制率．结果：５Ｆ，６Ｆ，Ａ均能够抑制ＤＮＡ拓扑异构酶Ⅰ，Ⅱ的活性，化合物６Ｆ作用细胞２４ｈ后，可升高Ｓ期和Ｇ２／Ｍ期细胞，同时降低Ｇ０／Ｇ１期细胞，低浓度６Ｆ（５７．８和１１５．６ｎｍｏｌ·Ｌ－１）与金雀异黄素（Ｇｅｎ）合用可增强它对ＨＬ－６０细胞的杀伤作用，ｑ＞１．１５．结论：５Ｆ，６Ｆ和Ａ明显抑制ＨＬ－６０细胞ＤＮＡ拓扑异构酶的活性；６Ｆ阻断细胞于Ｇ２／Ｍ期，增强Ｇｅｎ对ＨＬ－６０细胞的杀伤作用。

引用

页码：541 / 545

页数：5

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