四种转基因玉米新型质粒标准分子协同实验验证

被引:12
作者
沈愷琳 [1 ,2 ]
李想 [3 ]
王姝 [1 ]
张大兵 [1 ]
杨立桃 [1 ]
机构
[1] 上海交通大学生命科学技术学院陆伯勋食品安全中心转基因作物检测实验室
[2] 上海海洋大学水产与生命学院
[3] 上海出入境检验检疫局动植物与食品检验检疫技术中心
关键词
标准分子; 品系特异性; 协同实验; 转基因玉米;
D O I
暂无
中图分类号
S513 [玉米(玉蜀黍)];
学科分类号
0901 ;
摘要
构建了分别适于转基因玉米GA21、TC1507、T25和Bt11品系特异性双重PCR检测标准分子,并对其在定性PCR检测体系中的适用性进行了实验室间协同实验验证。对7家实验室返回结果的统计分析表明,标准分子pBt11、pTC、pT25和pGA分别对转基因玉米Bt11、TC1507、T25和GA21品系具有高特异性。4个标准分子在zSSⅡb和对应品系特异性序列单一PCR检测体系中检测下限均为50拷贝。因此,4个标准分子均可作为新型标准物质用于相应转基因玉米品系特异性定性检测。
引用
收藏
页码:90 / 95
页数:6
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