泛耐药鲍氏不动杆菌碳青霉烯酶基因型研究

被引：2

作者：

王倩

孙国全

欧阳金鸣

尚红

机构：

[1] 中国医科大学附属第一医院检验科

来源：

中华医院感染学杂志 | 2012年 / 22卷 / 20期

关键词：

鲍氏不动杆菌; 耐药; 基因;

D O I：

暂无

中图分类号：

R440 [];

学科分类号：

100208 ;

摘要：

目的研究医院泛耐药鲍氏不动杆菌(PDRAB)不同克隆的耐药性及碳青霉烯酶基因型特征,为有效治疗和控制PDRAB医院感染提供参考资料。方法应用脉冲场凝胶电泳(PFGE)技术对PDRAB进行分子分型,E试验法检测PDRAB各克隆最低抑菌浓度(MIC)值,聚合酶链反应(PCR)及核酸测序确定PDRAB各克隆碳青霉烯酶基因型。结果 90株PDRAB分子分型出现A、B、C、D、E 5种克隆,A、C克隆出现亚型,A、C、E克隆为主要流行株,分别占70.0%、7.8%、14.4%;各型克隆均对多黏菌素B敏感,部分流行克隆对米诺环素、阿米卡星敏感,其余抗菌药物均表现高水平耐药;A、C及C亚型克隆携带blaOXA-23、blaOXA-51和blaPER-1基因,A亚型、D克隆携带blaOXA-23和blaPER-1基因,B克隆携带blaOXA-51和blaPER-1基因,E克隆携带blaOXA-23、blaOXA-51和blaGIM-1基因,未发现携带blaOXA-24、blaOXA-58、blaIMP、blaVIM及blaSPM-1基因的PDRAB克隆。结论医院PDRAB流行克隆存在多样性,各克隆耐药表型及碳青霉烯酶基因型有所不同,产生OXA-23酶和PER-1金属酶是该地区PDRAB耐药的主要机制。

引用

页码：4431 / 4434

页数：4

共 6 条

[1]

Analysis of antibiotic resistance genes in multidrug-resistant Acinetobacter sp. isolates from military and civilian patients treated at the Walter Reed Army Medical Center. KM Hujer,AM Hujer,EA Hulten,S Bajaksouzian,JM Adams. Antimicrobial Agents and Chemotherapy . 2006

[2]

Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group. Iacono M,Villa L,Fortini D, et al. Antimicrobial Agents and Chemotherapy . 2008

[3]

Antimicrobial treatmentand clinical outcomes of carbapenem-resistant acineto-bacter baumannii ventilator-associated pneumonia. Chan JD,Graves JA,Dellit TH. JIntensive Care Med . 2010

[4]

Characterization of the natu-rally occurring oxacillinase of acinetobacter baumannii. Heritier C,Poirel L,Fournier PE,et al. Antimicrobial Agents and Chemotherapy . 2005

[5]

Horizontal gene transfer in a polyclonal outbreak of carbapenem-resistant Acinetobacter baumannii. Valenzuela JK,Lee Thomas,Partridge SR,et el. Journal of Clinical Microbiology . 2007

[6]

Outbreak of carbapenem-resistant Acinetobacter bau-mannii carrying the carbapenemase OXA-23 in a German university medical centre. Kohlenberg A,Higgins PG,et al. Medical Microbiology and Immunology . 2009

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