rhMG53蛋白对局灶性脑缺血再灌注损伤的保护作用研究

被引：7

作者：

姚勇刚 ^{[1
]}

张猛 ^{[2
]}

李美香 ^{[1
]}

曾敬 ^{[1
]}

曾春雨 ^{[1
]}

机构：

[1] 第三军医大学大坪医院野战外科研究所心血管内科,重庆市心血管病研究所

[2] 第三军医大学大坪医院野战外科研究所神经内科重症监护室

来源：

解放军医学杂志 | 2014年 / 06期

基金：

国家杰出青年科学基金; 国家自然科学基金重点项目;

关键词：

脑梗塞; 蛋白,MG53; 再灌注损伤;

D O I：

暂无

中图分类号：

R743.33 [脑栓塞];

学科分类号：

1002 ;

摘要：

目的观察重组人MG53蛋白对局灶性脑缺血再灌注(I/R)损伤的保护作用。方法采用大脑中动脉栓塞(MCAO)法建立SD大鼠脑IR模型,随机分为分假手术组、IR组、IR+rhMG53处理组(n=7),灌注24h后对各组大鼠进行神经系统Zea-Longa评分,然后处死大鼠,通过TTC染色观察脑梗死情况,HE染色观察大鼠脑组织病理改变。结果与IR组相比,IR+rhMG53处理组SD大鼠神经功能障碍明显改善,脑梗死面积缩小,脑组织坏死程度减轻,且保护作用与处理时间相关,外源性rhMG53对脑IR的有效保护时间窗在4h。结论外源性rhMG53对局灶性脑IR损伤具有明显的保护作用。

引用

页码：439 / 443

页数：5

共 11 条

[1]

Heart Disease and Stroke Statistics—2013 Update: A Report From the American Heart Association[J] . Alan S. Go,Dariush Mozaffarian,Véronique L. Roger,Emelia J. Benjamin,Jarett D. Berry,William B. Borden,Dawn M. Bravata,Shifan Dai,Earl S. Ford,Caroline S. Fox,Sheila Franco,Heather J. Fullerton,Cathleen Gillespie,Susan M. Hailpern,John A. Heit,Virginia J. Howard,Mark D. Huffman,Brett M. Kissela,Steven J. Kittner,Daniel T. Lackland,Judith H. Lichtman,Lynda D. Lisabeth,David Magid,Gregory M. Marcus,A

[2]

Rapid Conditioning With Oxygen Oscillation: Neuroprotection by Intermittent Normobaric Hyperoxia After Transient Focal Cerebral Ischemia in Rats[J] . Changsuo Liu,John Weaver,Ke Jian Liu.Stroke . 2012 (1)

[3]

Dysferlin, Annexin A1, and Mitsugumin 53 Are Upregulated in Muscular Dystrophy and Localize to Longitudinal Tubules of the T-System With Stretch[J] . Leigh B. Waddell,Frances A. Lemckert,Xi F. Zheng,Jenny Tran,Frances J. Evesson,Joanne M. Hawkes,Angela Lek,Neil E. Street,Peihui Lin,Nigel F. Clarke,Andrew P. Landstrom,Michael J. Ackerman,Noah Weisleder,Jianjie Ma,Kathryn N. North,Sandra T. Cooper.Journal of Neuropathology and Experimental Neurology . 2011 (4)

[4] Cardioprotection of Ischemia/Reperfusion Injury by Cholesterol-Dependent MG53-Mediated Membrane Repair [J].

Wang, Xianhua ;

Xie, Wenjun ;

Zhang, Yi ;

Lin, Peihui ;

Han, Liang ;

Han, Peidong ;

Wang, Yanru ;

Chen, Zheng ;

Ji, Guangju ;

Zheng, Ming ;

Weisleder, Noah ;

Xiao, Rui-Ping ;

Takeshima, Hiroshi ;

Ma, Jianjie ;

Cheng, Heping .

CIRCULATION RESEARCH, 2010, 107 (01) :76-U155

[5]

Calcium/calmodulin-dependent kinase II facilitated GluR6 subunit serine phosphorylation through GluR6-PSD95-CaMKII signaling module assembly in cerebral ischemia injury[J] . Jing Xu,Zhi-An Liu,Dong-Sheng Pei,Tie-Jun Xu.Brain Research . 2010

[6] MG53 Constitutes a Primary Determinant of Cardiac Ischemic Preconditioning [J].

Cao, Chun-Mei ;

Zhang, Yan ;

Weisleder, Noah ;

Ferrante, Christopher ;

Wang, Xianhua ;

Lv, Fengxiang ;

Zhang, Yi ;

Song, Ruisheng ;

Hwang, Moonsun ;

Jin, Li ;

Guo, Jiaojiao ;

Peng, Wei ;

Li, Geng ;

Nishi, Miyuki ;

Takeshima, Hiroshi ;

Ma, Jianjie ;

Xiao, Rui-Ping .

CIRCULATION, 2010, 121 (23) :2565-U74

[7] Mitsugumin 53-mediated maintenance of K+ currents in cardiac myocytes [J].

Masumiya, Haruko ;

Asaumi, Yasuhide ;

Nishi, Miyuki ;

Minamisawa, Susumu ;

Adachi-Akahane, Satomi ;

Yoshida, Morikatsu ;

Kangawa, Kenji ;

Ito, Kenta ;

Kagaya, Yutaka ;

Yanagisawa, Teruyuki ;

Yamazaki, Tetsuo ;

Ma, Jianjie ;

Takeshima, Hiroshi .

CHANNELS, 2009, 3 (01) :6-11

[8] Akt in ischemia and reperfusion [J].

Mullonkal, Carolyn J. ;

Toledo-Pereyra, Luis H. .

JOURNAL OF INVESTIGATIVE SURGERY, 2007, 20 (03) :195-203

[9] Reactive oxygen species plasmatic levels in ischemic stroke [J].

Laura Nanetti ;

Ruja Taffi ;

Arianna Vignini ;

Cinzia Moroni ;

Francesca Raffaelli ;

Tiziana Bacchetti ;

Mauro Silvestrini ;

Leandro Provinciali ;

Laura Mazzanti .

Molecular and Cellular Biochemistry, 2007, 303 :19-25

[10] Molecular biology of apoptosis in ischemia and reperfusion [J].

Lopez-Neblina, F ;

Toledo, AH ;

Toledo-Pereyra, LH .

JOURNAL OF INVESTIGATIVE SURGERY, 2005, 18 (06) :335-350

← 1 2 →