表没食子儿茶素促进Nrf2的核转位减轻Aβ25-35对SH-SY5Y细胞的损伤

被引：2

作者：

张雯 ^{[1
]}

宋俊科 ^{[1
]}

朱晓瑜 ^{[2
]}

杨海光 ^{[1
]}

王海港 ^{[1
]}

许启泰 ^{[2
]}

杜冠华 ^{[1
]}

机构：

[1] 中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室药物靶点研究与新药筛选北京市重点实验室

[2] 海南绿槟榔科技发展有限公司

来源：

中国药理学通报 | 2019年 / 35卷 / 10期

关键词：

β淀粉样蛋白; 人神经母细胞瘤细胞; 表没食子儿茶素; 核因子E2相关因子2; 氧化应激; 神经保护;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的研究表没食子儿茶素（epigallocatechin,EGC）对Aβ25-35损伤SH-SY5Y细胞的保护作用及机制。方法采用Aβ25-35损伤SH-SY5Y细胞,MTT和LDH法,考察EGC对SH-SY5Y细胞活力的影响。应用DCFH-DA荧光探针检测细胞内ROS水平,通过测定细胞内SOD、GSH-Px和MDA活性,考察细胞氧化应激水平。Western blot测定细胞中Nrf2、NQO1、HO-1、Prdx6和Trx1蛋白含量。结果 Aβ25-35损伤SH-SY5Y细胞,导致细胞存活率明显降低。5、10、20μmol·L-1 EGC能够减轻Aβ25-35诱导的SH-SY5Y细胞损伤及LDH释放。而且EGC能够减少Aβ25-35诱导的细胞内ROS水平的增加,减轻氧化应激损伤。同时,EGC能够明显增强Aβ25-35损伤后Nrf2、NQO1、HO-1、Prdx6和Trx1的表达。结论EGC能够抑制Aβ25-35诱导的SH-SY5Y细胞损伤,通过促进Nrf2核转位,提高抗氧化水平,降低Aβ25-35神经毒性。

引用

页码：1393 / 1398

页数：6

共 12 条

[1]

Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects.[J].Seyed-Mohamad-Sadegh Mirahmadi;Alireza Shahmohammadi;Ali-Mohammad Rousta;Mohammad-Reza Azadi;Javad Fahanik-Babaei;Tourandokht Baluchnejadmojarad;Mehrdad Roghani.Cytokine.2018,

[2]

Nrf2-a Promising Therapeutic Target for Defensing Against Oxidative Stress in Stroke [J].

Zhang, Rongrong ;

Xu, Mengxue ;

Wang, Yu ;

Xie, Fei ;

Zhang, Gang ;

Qin, Xinyue .

MOLECULAR NEUROBIOLOGY, 2017, 54 (08) :6006-6017

[3]

Chinese herbal medicine for Alzheimer’s disease: Clinical evidence and possible mechanism of neurogenesis.[J].Wen-ting Yang;Xia-wei Zheng;Shuang Chen;Chun-shuo Shan;Qing-qing Xu;Jia-Zhen Zhu;Xiao-Yi Bao;Yan Lin;Guo-qing Zheng;Yan Wang.Biochemical Pharmacology.2017,

[4]

Oxidative stress and mitochondrial dysfunction-linked neurodegenerative disorders [J].

Islam, Md. Torequl .

NEUROLOGICAL RESEARCH, 2017, 39 (01) :73-82

[5]

Nrf2 transcriptional derepression from Keap1 by dietary polyphenols [J].

Bayele, Henry K. ;

Debnam, Edward S. ;

Srai, Kaila S. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2016, 469 (03) :521-528

[6]

Nrf2–ARE pathway: An emerging target against oxidative stress and neuroinflammation in neurodegenerative diseases.[J].Izaskun Buendia;Patrycja Michalska;Elisa Navarro;Isabel Gameiro;Javier Egea;Rafael León.Pharmacology and Therapeutics.2016,

[7]

Oxidative stress response and Nrf2 signaling in aging.[J].Hongqiao Zhang;Kelvin J.A. Davies;Henry Jay Forman.Free Radical Biology and Medicine.2015, PB

[8]

Leonurine protects brain injury by increased activities of UCP4; SOD; CAT and Bcl-2; decreased levels of MDA and Bax; and ameliorated ultrastructure of mitochondria in experimental stroke.[J].Haichao Liu;Xiangjian Zhang;Yuanyuan Du;Hui Ji;Shuya Li;Litao Li;Yinxue Xing;Xiaolin Zhang;Lipeng Dong;Chaohui Wang;Kang Zhao;Ye Ji;Xiaoyun Cao.Brain Research.2012,

[9]

In Salvia miltiorrhiza, phenolic acids possess protective properties against amyloid β-induced cytotoxicity, and tanshinones act as acetylcholinesterase inhibitors [J].

Zhou, Yongqiang ;

Li, Weize ;

Xu, Lei ;

Chen, Lvyi .

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2011, 31 (03) :443-452

[10]

Cinnamon polyphenols attenuate cell swelling and mitochondrial dysfunction following oxygen-glucose deprivation in glial cells.[J].Kiran S. Panickar;Marilyn M. Polansky;Richard A. Anderson.Experimental Neurology.2008, 2

← 1 2 →