鲤鱼微卫星分子标记的筛选

被引：87

作者：

魏东旺

楼允东

孙效文

沈俊宝

机构：

[1] 上海水产大学渔业学院!上海

[2] 中国水产科学院黑龙江水产研究所!哈尔滨

来源：

动物学研究 | 2001年 / 03期

关键词：

鲤鱼; 微卫星; 分子标记;

D O I：

暂无

中图分类号：

Q75 [分子遗传学];

学科分类号：

071007 ;

摘要：

A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA) 15 probe labelled at the 5' end with γ 32 P ATP.Sequencing of these clones was performed with automated sequencer,and 22 microsatellites were isolated.17 primers were designed based on unique sequences flanking each motif with the software Primer3.PCR on Cyprinus carpio haematopterus was carried out with these primers,and all gave expected bands.Annealing temperature of these primers was between 50℃ and 53℃.

引用

页码：238 / 241

页数：4