小麦游离小孢子培养研究进展

被引：12

作者：

王宏芝

机构：

[1] 北京市农林科学院植物细胞工程实验室北京

来源：

麦类作物学报 | 2002年 / 01期

基金：

北京市自然科学基金;

关键词：

小麦小孢子; 胚胎发生; 子房共培养; 胁迫处理;

D O I：

暂无

中图分类号：

S512.103.2 [];

学科分类号：

摘要：

小麦小孢子培养一直是国内外长期以来努力攻克的课题。值得高兴的是 ,近几年来国际上在这方面的研究进展很大 ,已成功地建立了小麦小孢子的培养体系 ,同时在克服基因型限制问题上也有了一定的进展。主要技术要点如下 :1.小孢子和未成熟子房共培养对胚胎发生是十分必要的 ;2 .通过对外植体进行胁迫处理 ,可以有效地诱导小孢子由配子体发育途径向孢子体发育途径转变 ;3.对分离的小孢子采用Percoll梯度离心方法进行分级分离 ,可以降低基因型对小孢子胚胎发生的限制作用。本文对这些方面进行了综述 ,以期为我国开展小麦小孢子研究作些前期准备工作

引用

页码：91 / 94

页数：4

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